Protein Info for SMc04460 in Sinorhizobium meliloti 1021

Annotation: glycogen phosphorylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 821 TIGR02093: glycogen/starch/alpha-glucan phosphorylases" amino acids 21 to 819 (799 residues), 1147.5 bits, see alignment E=0 PF00343: Phosphorylase" amino acids 109 to 819 (711 residues), 1035.7 bits, see alignment E=0

Best Hits

Swiss-Prot: 47% identical to PHSG_SYNY3: Glycogen phosphorylase (glgP) from Synechocystis sp. (strain PCC 6803 / Kazusa)

KEGG orthology group: K00688, starch phosphorylase [EC: 2.4.1.1] (inferred from 100% identity to smk:Sinme_2897)

Predicted SEED Role

"Glycogen phosphorylase (EC 2.4.1.1)" in subsystem Glycogen metabolism or Maltose and Maltodextrin Utilization (EC 2.4.1.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.4.1.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q92M15 at UniProt or InterPro

Protein Sequence (821 amino acids)

>SMc04460 glycogen phosphorylase (Sinorhizobium meliloti 1021)
MMTRLSTTELPQPALRSSEPSQLAVEILERLKYRIGKDPKVAKPHDWLTAAILVARDRIT
DKWMDSTRKTYSTGAKRVYYMSLEFLIGRMMRDAMTNLGLMDEMRDALASLGVDIDVVAA
LEPDAALGNGGLGRLAACFMESMATVDVPAYGYGIRYMHGLFRQQMADGWQVELPETWLA
HGNPWEFERRESSYEIGFGGSVETVNIDEEVQRYVWKPAERVIATAFDTPAVGWRATRVN
TLRLWAAQPIDPILLHAFNAGDHIGALRESNKAESLTRVLYPADATPAGQELRLRQEYFF
SSASLQDILRRHLQQYPDFTSLPDAVAIQLNDTHPAVSVAELVRLLTDVHGLDFEQAWDI
SRRTFSYTNHTLLPEALESWPVPLFERLLPRHMQIVYAINAKILIEARRERHATDEAIRN
ISLIDETGDRRVRMGNLAFVGSHSINGVSALHTELMKETVFADLHALYPDRINNKTNGIT
PRRWLMQCNPGLFGLIREAIGDEFMDNTEALQALDAFADKADFQEHFAAVKRANKVRLAK
LVQANLGIRIDPSAMFDIQIKRIHEYKRQLLNLIEAVALYDQIRSHPELDWVPRVKLFAG
KAAPSYHNAKLIIKLANDIARVINNDPAVRGLLKVVFVPNYNVSLAEVMVPAADLSEQIS
TAGMEASGTGNMKFALNGALTIGTLDGANVEMRDWVGEENIKIFGMTAEEVGKARAEGHN
PRAVIENSRELSQALDAIASGVFSPDDRNRFSGLVDGLYNHDWFMVAADFEAYAKAQREI
DQLWTTPSAWYSKAVRNTARMGWFSSDRTIRQYAGEIWRAG