Protein Info for SMc04449 in Sinorhizobium meliloti 1021

Annotation: bifunctional 2',3'-cyclic nucleotide 2'-phosphodiesterase/3'-nucleotidase periplasmic protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 657 signal peptide" amino acids 1 to 33 (33 residues), see Phobius details PF00149: Metallophos" amino acids 39 to 281 (243 residues), 45.7 bits, see alignment E=1.1e-15 PF02872: 5_nucleotid_C" amino acids 443 to 574 (132 residues), 65.4 bits, see alignment E=6.5e-22

Best Hits

KEGG orthology group: K01119, 2',3'-cyclic-nucleotide 2'-phosphodiesterase [EC: 3.1.4.16] (inferred from 100% identity to smk:Sinme_1527)

Predicted SEED Role

"2',3'-cyclic-nucleotide 2'-phosphodiesterase (EC 3.1.4.16)" in subsystem Purine conversions (EC 3.1.4.16)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.1.4.16

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q926C9 at UniProt or InterPro

Protein Sequence (657 amino acids)

>SMc04449 bifunctional 2',3'-cyclic nucleotide 2'-phosphodiesterase/3'-nucleotidase periplasmic protein (Sinorhizobium meliloti 1021)
MTALHPISRRTLIGGLAATSAFVVLHPFSTRAAANQAHLRIMETTDLHVHVFPYDYYADK
PNDTLGLARTASIVDAIRAEAANAVLVDNGDFLQGNPMGDYIAYKRGMKEGDMHPVIAAM
NVLGYDCGTLGNHEFNYGLDFMFNVINGANFPIVCANLTKGAMAAAPRQDELFLKPYVIL
DRKVKDGAGQEHPIRIGLIGFVPPQIMTWDAKNLEGKANARDIVKAAEAWVPQMREEGAD
IVIALSHSGIGQQAYAENLENASVPLAAIEGIDAIVTGHSHLDFPGPKFDGTPGVDNAKG
LISGKPGVMGGFWGSHLGLIDLLLERDGGQWRVIGSTSEARPIFRREEKKVIAEVGDKPE
VLAAAQKDHEATLAYVRTPVGKTSAPLYSYFALVADDPSVQIVSQAQTWYIREMLKDTEH
SDLPVLSAAAPFKAGGRGGAEYYTDVPAGDIAIKNVADLYLYPNTVQAVVITGEEVRNWL
EMSAGIFNRIAPGSVDASLINGDFPSYNFDVIDGVTYQIVLSQPAKYDKDGNVANPEASR
IRDLKFDGRPIDPKQRFVVATNNYRAGGGGNFPGIAGDKVVFVAPDTNRDVIVRYIVEQG
TINPSADANWTFAPQKDTSVLFDSGPRARQFLAEVKAVKIENAGDGSDGFARFRIKL