Protein Info for SMc04444 in Sinorhizobium meliloti 1021

Annotation: NAD-dependent formate dehydrogenase subunit alpha protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 959 PF13510: Fer2_4" amino acids 18 to 94 (77 residues), 88.6 bits, see alignment E=9.1e-29 PF10588: NADH-G_4Fe-4S_3" amino acids 101 to 140 (40 residues), 60.6 bits, see alignment (E = 3.3e-20) PF13237: Fer4_10" amino acids 173 to 232 (60 residues), 27.4 bits, see alignment 1.1e-09 PF12838: Fer4_7" amino acids 177 to 234 (58 residues), 33 bits, see alignment 2.7e-11 PF00037: Fer4" amino acids 218 to 235 (18 residues), 24 bits, see alignment (E = 1e-08) PF04879: Molybdop_Fe4S4" amino acids 249 to 302 (54 residues), 60.6 bits, see alignment 4.7e-20 TIGR01591: formate dehydrogenase, alpha subunit" amino acids 253 to 928 (676 residues), 968 bits, see alignment E=1e-295 PF00384: Molybdopterin" amino acids 305 to 734 (430 residues), 234.3 bits, see alignment E=8.5e-73 PF01568: Molydop_binding" amino acids 820 to 925 (106 residues), 104.5 bits, see alignment E=1.4e-33

Best Hits

KEGG orthology group: K00123, formate dehydrogenase, alpha subunit [EC: 1.2.1.2] (inferred from 90% identity to ara:Arad_4285)

Predicted SEED Role

"NAD-dependent formate dehydrogenase alpha subunit" in subsystem Formate hydrogenase

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.2.1.2

Use Curated BLAST to search for 1.2.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q92LN1 at UniProt or InterPro

Protein Sequence (959 amino acids)

>SMc04444 NAD-dependent formate dehydrogenase subunit alpha protein (Sinorhizobium meliloti 1021)
MSLIHEIDYGTPASKSEKLVTLTIDGREITVPEGTSIMRAAMEAGIEVPKLCASDMMDAF
GSCRLCLVEIDGRAGMPASCTTPVAAGISVSTQTQRLKDVRRGVMELYISDHPLDCLTCA
ANGDCELQDMAGAVGLRDVRYGYDGENHVTARNNGEINAKWAPKDESNPYFTYDPAKCIV
CSRCVRACEEVQGTFALTISGRGFDSRVSAGMNEDFVSSECVSCGACVQACPTATLTEKS
VIEIGQPEHSVVTTCAYCGVGCSFKAEMRGEELVRMVPWKDGQANRGHSCVKGRFAYGYS
NHRDRILNPMVREKVTDPWREVTWEEAFAHVASEFRRIQYQYGRDSVGGITSSRCTNEET
FLVQKLVRAGFGNNNVDTCARVCHSPTGYGLNQTFGTSAGTQDFDSVEHTDVAVIIGANP
TDGHPVFASRLKKRLRQGAKLIVIDPRRIDLVRSAHVEASYHLPLKPGTNVAILTAIAHV
IVTEGLANEAFIRERCDWSEFEDWAAFVAEPHHSPEATEAYTGVPPELVRGAARLYATGG
NGAIYYGLGVTEHSQGSTTVMAIANLAMLTGNIGRPGVGVNPLRGQNNVQGSCDMGSFPH
ELPGYRHISDDATREIFEKLWGVTLNHEPGLRIPNMLDAAVEGTFKGLYVQGEDILQSDP
DTKHVAAGLAAMECVVVHDLFLNETANYAHVFLPGSTFLEKDGTFTNAERRINRVRRVMR
PKNGYADWEVTQKMAQAMGLAWNYRHPSEIMDEIAATTPSFAMVSYDYLDKMGSVQWPCN
EKAPLGSPIMHVDGFVRGKGKFIRTEYVATDERTGPRFPLLLTTGRILSQYNVGAQTRRT
ENVVWHAEDRLEIHPHDAEQRGIRDGDWVRLASRSGDTTLRALITDRVAPGVVYTTFHHP
STQANVITTDFTDWATNCPEYKVTAVQVSPSNGPSDWQRDYDEQARQSRRIAGKLEAAE