Protein Info for SMc04400 in Sinorhizobium meliloti 1021

Annotation: oxidoreductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 333 PF01408: GFO_IDH_MocA" amino acids 2 to 121 (120 residues), 85.5 bits, see alignment E=4.7e-28 PF02894: GFO_IDH_MocA_C" amino acids 133 to 329 (197 residues), 77.3 bits, see alignment E=1.5e-25

Best Hits

Swiss-Prot: 100% identical to AFR_RHIME: 1,5-anhydro-D-fructose reductase (afr) from Rhizobium meliloti (strain 1021)

KEGG orthology group: None (inferred from 100% identity to sme:SMc04400)

MetaCyc: 86% identical to 1,5-anhydro-D-fructose reductase (Ensifer adhaerens)
1,5-anhydro-D-fructose reductase (1,5-anhydro-D-mannitol-forming). [EC: 1.1.1.292]

Predicted SEED Role

"GLUCOSE-FRUCTOSE OXIDOREDUCTASE (EC 1.1.99.28)" (EC 1.1.99.28)

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 1.1.99.28

Use Curated BLAST to search for 1.1.1.292 or 1.1.99.28

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q92KZ3 at UniProt or InterPro

Protein Sequence (333 amino acids)

>SMc04400 oxidoreductase (Sinorhizobium meliloti 1021)
MIRWGLIGASTIAREWVIGAIRAAGGEVVSVMSSSAERGEAYAAENGIAKAVTSVDDLVG
DPDVDAVYISTTNELHHGQALAAIRAGKHVLCEKPLAMNLNDGCEMVLKACEAGVVLGTN
HHLRNAATHRAMREAIAAGRIGRPIAARVFHAVYLPPHLQGWRLDKPEAGGGVILDITVH
DADTLRFVLNDDPIEAVAISHSAGMGKEGLEDGVMGVLRFRSGVIAQFHDAFTTKFAETG
LEVHGTAGSLIGRNVMTQRPVGTVVLRNEEGESELPLDHRNLYETAIAAFHSAIGGNGRP
SASGEDGVWSLATGLAVVKAAATGGAVEIETGL