Protein Info for SMc04398 in Sinorhizobium meliloti 1021

Annotation: enoyl-CoA hydratase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00378: ECH_1
  • amino acids 11 to 257 (247 residues), 174.2 bits, E=3.2e-55
• PF16113: ECH_2
  • amino acids 15 to 197 (183 residues), 106.1 bits, E=2.8e-34

Best Hits

Swiss-Prot: 35% identical to CRCH_SYNWW: Crotonyl-CoA hydratase (Swol_1936) from Syntrophomonas wolfei subsp. wolfei (strain DSM 2245B / Goettingen)

KEGG orthology group: K01692, enoyl-CoA hydratase [EC: 4.2.1.17] (inferred from 100% identity to smk:Sinme_3295)

MetaCyc: 37% identical to crotonase (Clostridium kluyveri)
RXN-11667 [EC: 4.2.1.150]

Predicted SEED Role

"3-hydroxybutyryl-CoA dehydratase (EC 4.2.1.55)" in subsystem Acetyl-CoA fermentation to Butyrate or Polyhydroxybutyrate metabolism (EC 4.2.1.55)

MetaCyc Pathways

• oleate β-oxidation (29/35 steps found)
• superpathway of glyoxylate cycle and fatty acid degradation (12/14 steps found)
• 1-butanol autotrophic biosynthesis (engineered) (21/27 steps found)
• adipate degradation (5/5 steps found)
• acetyl-CoA fermentation to butanoate (6/7 steps found)
• benzoyl-CoA biosynthesis (3/3 steps found)
• glycerol degradation to butanol (12/16 steps found)
• adipate biosynthesis (4/5 steps found)
• (2S)-ethylmalonyl-CoA biosynthesis (3/4 steps found)
• fatty acid β-oxidation I (generic) (5/7 steps found)
• L-isoleucine degradation I (4/6 steps found)
• pyruvate fermentation to butanol II (engineered) (4/6 steps found)
• fatty acid β-oxidation II (plant peroxisome) (3/5 steps found)
• fatty acid β-oxidation IV (unsaturated, even number) (3/5 steps found)
• glutaryl-CoA degradation (3/5 steps found)
• L-valine degradation I (5/8 steps found)
• pyruvate fermentation to hexanol (engineered) (7/11 steps found)
• 2-methyl-branched fatty acid β-oxidation (9/14 steps found)
• L-glutamate degradation V (via hydroxyglutarate) (6/10 steps found)
• methyl ketone biosynthesis (engineered) (3/6 steps found)
• propanoate fermentation to 2-methylbutanoate (3/6 steps found)
• phenylacetate degradation I (aerobic) (5/9 steps found)
• superpathway of Clostridium acetobutylicum acidogenic fermentation (5/9 steps found)
• valproate β-oxidation (5/9 steps found)
• pyruvate fermentation to butanol I (4/8 steps found)
• (8E,10E)-dodeca-8,10-dienol biosynthesis (6/11 steps found)
• superpathway of phenylethylamine degradation (6/11 steps found)
• benzoyl-CoA degradation I (aerobic) (3/7 steps found)
• fatty acid β-oxidation VI (mammalian peroxisome) (3/7 steps found)
• pyruvate fermentation to butanoate (3/7 steps found)
• benzoate biosynthesis III (CoA-dependent, non-β-oxidative) (1/5 steps found)
• gallate degradation III (anaerobic) (5/11 steps found)
• 3-hydroxypropanoate/4-hydroxybutanate cycle (10/18 steps found)
• 3-phenylpropanoate degradation (4/10 steps found)
• superpathway of Clostridium acetobutylicum solventogenic fermentation (6/13 steps found)
• benzoate biosynthesis I (CoA-dependent, β-oxidative) (3/9 steps found)
• ethylmalonyl-CoA pathway (4/11 steps found)
• superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation (8/17 steps found)
• L-glutamate degradation VII (to butanoate) (4/12 steps found)
• (4Z,7Z,10Z,13Z,16Z)-docosapentaenoate biosynthesis (6-desaturase) (2/13 steps found)
• L-tryptophan degradation III (eukaryotic) (3/15 steps found)
• docosahexaenoate biosynthesis III (6-desaturase, mammals) (2/14 steps found)
• crotonate fermentation (to acetate and cyclohexane carboxylate) (3/16 steps found)
• benzoate fermentation (to acetate and cyclohexane carboxylate) (3/17 steps found)
• toluene degradation VI (anaerobic) (3/18 steps found)
• Spodoptera littoralis pheromone biosynthesis (4/22 steps found)
• platensimycin biosynthesis (6/26 steps found)

KEGG Metabolic Maps

• Benzoate degradation via CoA ligation
• Biosynthesis of plant hormones
• Biosynthesis of unsaturated fatty acids
• Butanoate metabolism
• Caprolactam degradation
• Fatty acid elongation in mitochondria
• Fatty acid metabolism
• Geraniol degradation
• Limonene and pinene degradation
• Lysine degradation
• Propanoate metabolism
• Tryptophan metabolism
• Valine, leucine and isoleucine degradation
• alpha-Linolenic acid metabolism
• beta-Alanine metabolism

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.17, 4.2.1.55

Use Curated BLAST to search for 4.2.1.150 or 4.2.1.17 or 4.2.1.55

Sequence Analysis Tools

See Q92KZ5 at UniProt or InterPro

Protein Sequence (259 amino acids)

>SMc04398 enoyl-CoA hydratase (Sinorhizobium meliloti 1021)
MADERIRVDFEGPIAIVTVARPEKLNAFDIDMLKALDSACDTVEARRDLRAVILTGEGKA
FSAGGDIKAWGGMEPAAFGHDWVRFGHRVFERLATLRMPVIAALNGHALGGGLELAAAAD
IRLAEKQVKIGLPETSLGMVPGWSGTQRLVARFGAQIVRRMVLGGEMFSAAEARAEGLID
AVVETGAVMQAARDYAARVAGRGPAALEISKLMIASASGEDKGAAVEALGSILVAKTGDL
KEGVAAFSEKREARFKGEW