Protein Info for SMc04255 in Sinorhizobium meliloti 1021

Annotation: beta-mannosidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 831 PF22666: Glyco_hydro_2_N2" amino acids 26 to 195 (170 residues), 161.4 bits, see alignment E=3.1e-51 PF17753: Ig_mannosidase" amino acids 757 to 808 (52 residues), 53.4 bits, see alignment 2.5e-18

Best Hits

KEGG orthology group: K01192, beta-mannosidase [EC: 3.2.1.25] (inferred from 100% identity to sme:SMc04255)

Predicted SEED Role

"Beta-mannosidase (EC 3.2.1.25)" in subsystem Mannose Metabolism (EC 3.2.1.25)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.2.1.25

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q92P68 at UniProt or InterPro

Protein Sequence (831 amino acids)

>SMc04255 beta-mannosidase (Sinorhizobium meliloti 1021)
MHEWTMEMPSETPNRDAVRIDLSGDWLLAAADNSHALTIALPGDVHSALQRAGIIADPYR
GRNEADVQWVARKDWVLERTVVIDADDLNGGWYLDFESIDTVASVYVNDELVLQAENCFR
RYRPDVSGAIVAGENRIRIVLHSAIAEGARRQAAQPFYVPYHDGNSPIANGNMLRKPQCH
FGWDWNIAIAPLGIYGSLALCRLETARIEHVTTRQMWLDDGSVDLQVTVQLFAREPGIVP
VHFMLDGVRERLDCAVGAGETRLTHVFTVQEPRRWWPAGSGDQAFSTLKVEVPGETVTRQ
IGFRTIELVTDKDEAGSRFAFRVNGREIFCRGANWIPADALMSRVTPESVEDLLRSAVDA
NMNMIRVWGGGFYEPDWFYDLCDRLGLLVWQDFMFACNLYPSTPDFLENVAAEVDYQVRR
LSTHPSLALWCGDNELVGALTWFEESRRDRDRYLVSYDRLNRTVEAVMKQACPEAIWWPS
SPSVGYLDFGDAWHADGAGDMHYWSVWHENKSFDNYRTVRPRFCSEFGFQSYTSMQVIRQ
FAEAHDLNIASPVMEAHQKNAGGNERIAGTMFRYFRFPKDFPSFVYLSQIQQGLAIRTAV
DYWRSLKPHCMGTLYWQLNDTWPVASWSSLDYGGHWKAMHYMARRFFQPVAVAAIPSADG
REIAFSMVNDTAEPATVELQAFLVSLDGNRQRLLAAAGTCPADRASTLVTVAAKDIPPDT
LLFWSFEASNGATGEGHYAHGTYKALDLVPSGLTIEAVPRPDGSFDVAVTAAGLALHVMI
EADVEGRYSDNAFDLTAGETKSIRFTPRIALDAGASPRFSAYDLESCQGKG