Protein Info for SMc04128 in Sinorhizobium meliloti 1021

Annotation: heavy metal transporting ATPase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 743 transmembrane" amino acids 137 to 155 (19 residues), see Phobius details amino acids 161 to 179 (19 residues), see Phobius details amino acids 186 to 204 (19 residues), see Phobius details amino acids 210 to 229 (20 residues), see Phobius details amino acids 360 to 380 (21 residues), see Phobius details amino acids 391 to 415 (25 residues), see Phobius details amino acids 698 to 717 (20 residues), see Phobius details amino acids 723 to 742 (20 residues), see Phobius details PF00403: HMA" amino acids 10 to 55 (46 residues), 48.3 bits, see alignment 1.6e-16 TIGR01512: cadmium-translocating P-type ATPase" amino acids 190 to 497 (308 residues), 332.3 bits, see alignment E=1e-102 TIGR01525: heavy metal translocating P-type ATPase" amino acids 190 to 741 (552 residues), 585.1 bits, see alignment E=3.6e-179 TIGR01511: copper-translocating P-type ATPase" amino acids 208 to 741 (534 residues), 406.3 bits, see alignment E=5e-125 TIGR01494: HAD ATPase, P-type, family IC" amino acids 213 to 716 (504 residues), 280.4 bits, see alignment E=4.2e-87 PF00122: E1-E2_ATPase" amino acids 241 to 421 (181 residues), 191.8 bits, see alignment E=1.2e-60 PF00702: Hydrolase" amino acids 437 to 653 (217 residues), 112.3 bits, see alignment E=6e-36

Best Hits

KEGG orthology group: K01534, Cd2+/Zn2+-exporting ATPase [EC: 3.6.3.3 3.6.3.5] (inferred from 100% identity to smk:Sinme_3455)

Predicted SEED Role

"Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4)" (EC 3.6.3.3, EC 3.6.3.4, EC 3.6.3.5)

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.3, 3.6.3.4, 3.6.3.5

Use Curated BLAST to search for 3.6.3.3 or 3.6.3.4 or 3.6.3.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q92T56 at UniProt or InterPro

Protein Sequence (743 amino acids)

>SMc04128 heavy metal transporting ATPase (Sinorhizobium meliloti 1021)
MTGIFRETRFRVDGMDCASCAAKIDTAVRRVAGVEDVNVSVAAGTMTVRHAARDDIGAQV
MRKVGVLGYGLSPLDVAAERQPAQSEHACCGHDHAAGSAHGEASHDHGHSTALPASTKPS
SRFTAPLPWWRTAKGKLTLACGVALASAYAIGQFVPATEPWIFTLAMLVGLLPIARRALM
AALSGTLFSIEMLMTIAAAGAVFIGAGEEAAMVVFLFLIGELLEGVAAGKARASIQALTA
LVPKSALLEENGRTVEVPAESLAPGAIVLVRPGDRLPADGIIVSGESSVDEAPVTGESTP
VLKEAGANVFAGTVNGDGALRVRVTAAAADNTIARVIRLVEEAQEKKAPTERFIDRFSRY
YTPGVVLVAALVAVIPPLFFGGLWQDWIYKGLALLLIGCPCALVISTPAAIAASLSAGAR
RGLLIKGGAVLENLGRITAVAFDKTGTLTEGRPKLTDIVGFGRSEAEILGYAAALEQGSS
HPLAHAVLSRAEADGLALLPVEGARAIGGKGVAASADGVELFLGSPEAARERAPLAAEDL
ARIETLQGEGKTVSVLVVGGRAAGALAMRDEPRADAAAGLRALADQGLRVVMLTGDNRAT
AEAIAGRLGGIEAHAGLLPEDKQRIVSRLRAEGLVVAKVGDGINDAPALAAADVGIAVGG
GTDVALETADAASLHARVSDVAAMVKLSRVTMRNIHQNIAIALGLKAVFLATTIAGVTGL
WPAILADTGATVLVTINALRLLR