Protein Info for SMc03978 in Sinorhizobium meliloti 1021

Annotation: transketolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF13292: DXP_synthase_N
  • amino acids 3 to 194 (192 residues), 25.8 bits, E=1.6e-09
• PF00456: Transketolase_N
  • amino acids 7 to 330 (324 residues), 475.6 bits, E=1.9e-146
• TIGR00232: transketolase
  • amino acids 10 to 657 (648 residues), 834.2 bits, E=3.7e-255
• PF00676: E1_dh
  • amino acids 112 to 244 (133 residues), 30.3 bits, E=5.6e-11
• PF02779: Transket_pyr
  • amino acids 352 to 522 (171 residues), 183.2 bits, E=8.7e-58
• PF02780: Transketolase_C
  • amino acids 545 to 650 (106 residues), 44.6 bits, E=3.3e-15

Best Hits

Swiss-Prot: 66% identical to TKT1_XANFL: Transketolase 1 (tkt) from Xanthobacter flavus

KEGG orthology group: K00615, transketolase [EC: 2.2.1.1] (inferred from 100% identity to smk:Sinme_2818)

MetaCyc: 49% identical to transketolase 2 (Escherichia coli K-12 substr. MG1655)
Transketolase. [EC: 2.2.1.1]; 2.2.1.1 [EC: 2.2.1.1]

Predicted SEED Role

"Transketolase (EC 2.2.1.1)" in subsystem Calvin-Benson cycle or Pentose phosphate pathway (EC 2.2.1.1)

MetaCyc Pathways

• photosynthetic 3-hydroxybutanoate biosynthesis (engineered) (22/26 steps found)
• Bifidobacterium shunt (14/15 steps found)
• Rubisco shunt (10/10 steps found)
• superpathway of glucose and xylose degradation (15/17 steps found)
• Calvin-Benson-Bassham cycle (12/13 steps found)
• formaldehyde assimilation III (dihydroxyacetone cycle) (11/12 steps found)
• pentose phosphate pathway (8/8 steps found)
• 1-butanol autotrophic biosynthesis (engineered) (21/27 steps found)
• pentose phosphate pathway (non-oxidative branch) I (5/5 steps found)
• ethene biosynthesis V (engineered) (19/25 steps found)
• pentose phosphate pathway (partial) (3/3 steps found)
• oxygenic photosynthesis (13/17 steps found)
• pentose phosphate pathway (non-oxidative branch) II (5/6 steps found)
• formaldehyde assimilation II (assimilatory RuMP Cycle) (7/9 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of ansamycins
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Carbon fixation in photosynthetic organisms
• Pentose phosphate pathway

Isozymes

Compare fitness of predicted isozymes for: 2.2.1.1

Use Curated BLAST to search for 2.2.1.1

Sequence Analysis Tools

See Q92M82 at UniProt or InterPro

Protein Sequence (660 amino acids)

>SMc03978 transketolase (Sinorhizobium meliloti 1021)
MISREKHDRMANAIRFLSMDAVEKANSGHPGLPMGAADIATVLFTRYLSFDPKNPAWPNR
DRFVLSAGHGSMLLYSLLYLTGYDDITIDEIKNFRQLGSRTAGHPEYGHAAGIETTTGPL
GQGIANSVGMAIAERKLREEFGSELMEHYTYVLAGDGCLMEGISQEAIALAGHLKLNKLI
VFWDDNNISIDGPISIADSTDQHARFRASKWHTIAVDGHDPEAIAAAIEEAHKSDKPTMI
ACKTVIGFGAPNKAGTHKVHGSPLGAEEIAATRKALGWEAEAFTVPSDVLDAWRIAGLRS
AKARKEWEERLEAAEAEKKAQFVRRFSGELEGSLASAIGAYKQKLAETKPSPATRKASED
ALEVINGVLAETIGGSADLTGSNNTKTSQTHSITPDDFSGRYIHYGVREHGMAAAMNGMA
LHGGLIPYSGGFLIFSDYCRPSIRLAALMGIRVIHVLTHDSIGLGEDGPTHQPVEHMAAL
RAIPNLLMFRPADATETAECWQLALENRKRPSGLALTRQNLMAVRTEYEEQNLCARGAYD
LISASDAQVTIFATGSEVEIAVKACQTLTAKGIATRVVSVPCFELFAEQSEDYQQAIIGT
SPVKIAVEAGIRQGWDHFIGNDGIFIGMSSFGASGPYKDLYKHFGITPEVVVASAEAKLS