Protein Info for SMc03878 in Sinorhizobium meliloti 1021

Annotation: acetoacetyl-CoA reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 241 signal peptide" amino acids 1 to 16 (16 residues), see Phobius details PF00106: adh_short" amino acids 3 to 190 (188 residues), 192.9 bits, see alignment E=6.6e-61 TIGR01829: acetoacetyl-CoA reductase" amino acids 3 to 240 (238 residues), 390.6 bits, see alignment E=1.5e-121 PF08659: KR" amino acids 5 to 169 (165 residues), 57.1 bits, see alignment E=3.3e-19 PF13561: adh_short_C2" amino acids 12 to 237 (226 residues), 202.4 bits, see alignment E=1.3e-63

Best Hits

Swiss-Prot: 100% identical to PHAB_RHIME: Acetoacetyl-CoA reductase (phaB) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K00023, acetoacetyl-CoA reductase [EC: 1.1.1.36] (inferred from 100% identity to sme:SMc03878)

MetaCyc: 87% identical to acetoacetyl-CoA reductase subunit (Zoogloea ramigera)
Acetoacetyl-CoA reductase. [EC: 1.1.1.36]; 1.1.1.36 [EC: 1.1.1.36]

Predicted SEED Role

"Acetoacetyl-CoA reductase (EC 1.1.1.36)" in subsystem Acetyl-CoA fermentation to Butyrate or Polyhydroxybutyrate metabolism or Serine-glyoxylate cycle (EC 1.1.1.36)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.1.36

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P50205 at UniProt or InterPro

Protein Sequence (241 amino acids)

>SMc03878 acetoacetyl-CoA reductase (Sinorhizobium meliloti 1021)
MSRVALVTGGSRGIGAAICVALKAAGYKVAANYAGNDERAKAFEQESGIPVYKWDVSSYQ
ACVDGIARVEADLGPVDILVNNAGITRDAMFHKMTPEQWGEVIGTNLTGVFNMTHPLWSG
MRDRGFGRIVNISSINGQKGQMGQVNYSAAKAGDLGLTKALAQEGAAKGITVNAICPGYI
GTEMVRAVPEKVLNERIIPQIPVGRLGEPEEVARCVVFLASDDAGFITGSTISANGGQYF
A