Protein Info for SMc03869 in Sinorhizobium meliloti 1021

Annotation: thiamine transporter substrate binding subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 342 signal peptide" amino acids 1 to 30 (30 residues), see Phobius details TIGR01276: thiamin/thiamin pyrophosphate ABC transporter, thiamin/thiamin pyrophospate-binding protein" amino acids 20 to 341 (322 residues), 398.3 bits, see alignment E=2.6e-123 TIGR01254: ABC transporter periplasmic binding protein, thiB subfamily" amino acids 30 to 335 (306 residues), 386.2 bits, see alignment E=1.1e-119 PF13531: SBP_bac_11" amino acids 51 to 287 (237 residues), 58.8 bits, see alignment E=1.4e-19 PF13416: SBP_bac_8" amino acids 51 to 284 (234 residues), 71.1 bits, see alignment E=2.8e-23 PF01547: SBP_bac_1" amino acids 52 to 284 (233 residues), 75.7 bits, see alignment E=1.3e-24 PF13343: SBP_bac_6" amino acids 86 to 303 (218 residues), 69.1 bits, see alignment E=8.4e-23

Best Hits

Swiss-Prot: 68% identical to THIB_BRUSU: Thiamine-binding periplasmic protein (thiB) from Brucella suis biovar 1 (strain 1330)

KEGG orthology group: K02064, thiamine transport system substrate-binding protein (inferred from 100% identity to sme:SMc03869)

MetaCyc: 57% identical to thiamine ABC transporter periplasmic binding protein (Escherichia coli K-12 substr. MG1655)
ABC-32-RXN [EC: 7.6.2.15]

Predicted SEED Role

"Thiamin ABC transporter, substrate-binding component" in subsystem Thiamin biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.6.2.15

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q92L33 at UniProt or InterPro

Protein Sequence (342 amino acids)

>SMc03869 thiamine transporter substrate binding subunit (Sinorhizobium meliloti 1021)
MSSSLHRKILGRLAIAAASIAAFSVSAAAAEKTLTVYTYESFITEWGPGAKVAEAFEKTC
DCKVDYVGVADGVELLTRLKLEGQGSKADIVLGLDTNLVAEAKATGFFAPHGADTASVKV
PGGFSDDTFIPYDYGHFAVVYDTEALKDPPKSLKELVEGDPSQKIVIEDPRTSTPGLGLL
LWVKSVYGDKAGEAWAKLKDRVLTVTPGWSEAYGLFTKGEAPMVLSYTTSPAYHMVAENT
ERYQAAPFAEGHYIQIEVAALTKNAKDPELARNFLTFMIGPEFQSIIPTTNWMMPVTATK
EPLPEAFGKLVDPQKTFLIPSGEVAASRKAWIDEWLAAMSRN