Protein Info for SMc03867 in Sinorhizobium meliloti 1021

Annotation: hypothetical protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01378: thiamine pyrophosphokinase
  • amino acids 7 to 206 (200 residues), 146.7 bits, E=3.2e-47
• PF04263: TPK_catalytic
  • amino acids 29 to 126 (98 residues), 63.8 bits, E=1.4e-21
• PF04265: TPK_B1_binding
  • amino acids 150 to 202 (53 residues), 30.8 bits, E=1.9e-11

Best Hits

KEGG orthology group: K00949, thiamine pyrophosphokinase [EC: 2.7.6.2] (inferred from 100% identity to smk:Sinme_3247)

Predicted SEED Role

"Thiamin pyrophosphokinase (EC 2.7.6.2)" in subsystem PnuC-like transporters or Thiamin biosynthesis (EC 2.7.6.2)

MetaCyc Pathways

• thiamine diphosphate salvage III (1/1 steps found)
• thiamine diphosphate biosynthesis III (Staphylococcus) (2/3 steps found)
• thiamine diphosphate biosynthesis IV (eukaryotes) (2/3 steps found)
• superpathway of thiamine diphosphate biosynthesis III (eukaryotes) (4/7 steps found)
• thiamine diphosphate salvage IV (yeast) (4/7 steps found)
• thiamine diphosphate formation from pyrithiamine and oxythiamine (yeast) (4/8 steps found)

KEGG Metabolic Maps

• Thiamine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.6.2

Sequence Analysis Tools

See Q92L35 at UniProt or InterPro

Protein Sequence (216 amino acids)

>SMc03867 hypothetical protein (Sinorhizobium meliloti 1021)
MTRSPFTILLGGALTPTDRLARQLEGSRFVAADGGMRHARTLGVVPDLWVGDFDSTDEAL
LAEFAGVPRERHPAAKNATDGEIAVEAALERGATSLVFAGGLGGARSDHAFLHLLGTVAL
AESGVAVMMTSGEEEAYPLLPGSQEIELPKGSLFSILGFADLDGLSIENVRYPLKDFHLP
FGSSRTISNIADGTVRFTLKSGRAVILARPYDLSGA