Protein Info for SMc03855 in Sinorhizobium meliloti 1021

Annotation: hypothetical protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 424 TIGR00089: radical SAM methylthiotransferase, MiaB/RimO family" amino acids 4 to 385 (382 residues), 328.5 bits, see alignment E=5.9e-102 PF00919: UPF0004" amino acids 4 to 90 (87 residues), 68.6 bits, see alignment E=4e-23 TIGR01579: MiaB-like tRNA modifying enzyme" amino acids 7 to 390 (384 residues), 427.5 bits, see alignment E=7e-132 PF04055: Radical_SAM" amino acids 141 to 314 (174 residues), 82.6 bits, see alignment E=4e-27

Best Hits

Swiss-Prot: 56% identical to MTAB_RICPR: Threonylcarbamoyladenosine tRNA methylthiotransferase MtaB (mtaB) from Rickettsia prowazekii (strain Madrid E)

KEGG orthology group: None (inferred from 100% identity to smk:Sinme_3235)

Predicted SEED Role

"tRNA-t(6)A37 methylthiotransferase"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q92L47 at UniProt or InterPro

Protein Sequence (424 amino acids)

>SMc03855 hypothetical protein (Sinorhizobium meliloti 1021)
MSGVEVITFGCRLNTYESEVMRAEAEKAGLNNAVLVNTCAVTAEAVRQARQAIRRARREN
PHARIIVTGCAAQTEKETFAEMAEVDAVLGNEEKLASVSYRSLPDFGVSAEEKLRVNDIM
SVRATAPQMVKHIDGHVRAFIQVQNGCDHRCTFCIIPYGRGNSRSVPMGAVVDQARRLAE
SGYREIVLTGVDATSYGADLPGTPTLGLLAKTLLKQVPEILRLRLSSIDSIEADGHLLDL
IAEEPRFMPHLHLSLQHGDDLILKRMKRRHSSADARAFCDEVRRLRPGISLGADMIAGFP
TETEPMFENAMRLAEDCGIAHLHVFPYSPRPGTPAARMPQLDRALVKERAARLRAKGAEL
HAGHLEGMIGSSQTILVEMNGLAHTENFTLVDAAGLEPRSLVAVGITGHNGKHLTIERKQ
MAAA