Protein Info for SMc03831 in Sinorhizobium meliloti 1021

Annotation: hypothetical protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 411 TIGR00048: 23S rRNA (adenine(2503)-C(2))-methyltransferase" amino acids 25 to 391 (367 residues), 393.4 bits, see alignment E=4.3e-122 PF21016: RlmN_N" amino acids 52 to 90 (39 residues), 43.2 bits, see alignment 2.6e-15 PF04055: Radical_SAM" amino acids 200 to 322 (123 residues), 42.1 bits, see alignment E=1.1e-14

Best Hits

Swiss-Prot: 100% identical to RLMN_RHIME: Dual-specificity RNA methyltransferase RlmN (rlmN) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K06941, ribosomal RNA large subunit methyltransferase N [EC: 2.1.1.-] (inferred from 100% identity to sme:SMc03831)

Predicted SEED Role

"Ribosomal RNA large subunit methyltransferase N (EC 2.1.1.-)" in subsystem Conserved gene cluster associated with Met-tRNA formyltransferase (EC 2.1.1.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.-

Use Curated BLAST to search for 2.1.1.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q92L68 at UniProt or InterPro

Protein Sequence (411 amino acids)

>SMc03831 hypothetical protein (Sinorhizobium meliloti 1021)
MAATEILNRADIVKAPQVRLAPQPEKPSLIGLLRDDIAKLLAEKGVPERQVKMRVSQLWH
WLYVRGVSDFDEMSNVSKDMREMLKEHFTIARPDIVEEQVSGDGTRKWLLRFPPRGAGRP
VEIETVYIPEEGRGTLCISSQVGCTLTCSFCHTGTQKLVRNLTAEEILSQLLLARDRLGD
FPERDTPQGAIVPAEGRKITNIVMMGMGEPLYNFENVKTALLIASDGDGLSLSKRRITLS
TSGIVPEIYRTGEEIGVMLAISLHAVRDDLRDMLVPINKKYPLKQLMEACRAYPGLSNAR
RITFEYVMLKDVNDSLEDAKELVKLLKGIPAKINLIPFNPWPGTNYQCSDWEQIEKFADF
INQAGYASPIRTPRGRDILAACGQLKSESERMRKVDRLAFEAMMIANHGED