Protein Info for SMc03788 in Sinorhizobium meliloti 1021

Annotation: DNA polymerase III subunit alpha

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1116 TIGR00594: DNA polymerase III, alpha subunit" amino acids 7 to 1008 (1002 residues), 477.1 bits, see alignment E=6.6e-147 PF02811: PHP" amino acids 12 to 124 (113 residues), 29.2 bits, see alignment E=2.7e-10 PF07733: DNA_pol3_alpha" amino acids 279 to 533 (255 residues), 293.6 bits, see alignment E=3.8e-91 PF17657: DNA_pol3_finger" amino acids 536 to 705 (170 residues), 182.7 bits, see alignment E=8.7e-58 PF14579: HHH_6" amino acids 779 to 885 (107 residues), 71.1 bits, see alignment E=1.9e-23 PF01336: tRNA_anti-codon" amino acids 973 to 1048 (76 residues), 43.9 bits, see alignment 4.7e-15

Best Hits

Swiss-Prot: 100% identical to DNE21_RHIME: Error-prone DNA polymerase 1 (dnaE2-1) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K14162, error-prone DNA polymerase [EC: 2.7.7.7] (inferred from 100% identity to smk:Sinme_3167)

Predicted SEED Role

"Error-prone repair homolog of DNA polymerase III alpha subunit (EC 2.7.7.7)" (EC 2.7.7.7)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.7

Use Curated BLAST to search for 2.7.7.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q92LA6 at UniProt or InterPro

Protein Sequence (1116 amino acids)

>SMc03788 DNA polymerase III subunit alpha (Sinorhizobium meliloti 1021)
MSADAVFCELGARTNFSFLEGAAPAEEMVVFAKKAGLAGLGIADRNSVAGVVRAHAKAKV
EGYPFQPGARLVFADGTPDILAYPKNRRGWGHLCRLLSAGNLRSKKGDCTLHLADLLEWQ
EELLLIVMQGEGRPEPESLEVLLGTLKEHAGNRLYLGLAPHYDGFDRHDFAVLAAIARKA
GIGLLATNDALYHDPHYRPLADVVTSIREHVPIAGAGFLLQKNAERHLKGPREMARLFSD
YPEAIANTRKFFRELAFSLDELSHQYPDENADGETPAESLRRLVAEGAAERYPEGVPEKV
MRQIDYELELIHDKKYEPYFLTVHKLVKFARSVNILCQGRGSAANSSVCFCLGITDVDPQ
KFTLLFDRFLSKDRDEPPDIDVDFEHERREEVIQYIYRTYGKEHAGLTAAVISYRSRSAG
REVAKAFGLSEDVQSALVSSIWGWGTSPFTEEQAKGAGLDAADPLTRRVLAYASLLMNFP
RHLSQHVGGFVITRDRLDEVVPIMNTAMPDRYMIEWDKDDLDELKILKVDVLALGMLTCL
AKGFKLLEAHYGEPITLAEIYQDHRDAVYDMICRADTVGVFQIESRAQMSMLPRLQPREM
YDLVIEVAIVRPGPIQGNMVHPYLKRREAQRRGEAVVYPSPELKAVLERTLGVPLFQEQA
MQIAITAAGFSPSEADRLRRAMATFKRTGTIHTFERKMVEGMVANDYEREFAERCFNQIK
GFGEYGFPESHAASFASLVYASAWLKTYYPDIFCAALLNAQPMGFYAPAQLVRDAREHGV
RMLPVDINHSDWDALLEGEGAFDKNAVHPRHASMREVIKTRKAVRLGFRLVKGLKQTDMK
ALVARRGEGYRSVHDLWLRSGLSRSVLERLADADAFRSIGLDRRAALWAVKALDEQSAVE
RLPLFEGAGSDDLQIEPKVALPDMPAGEQVIHDYRTLTLSLKAHPVSFMREDFSRRGILR
SRDLAATATGRWVTVAGLVLVRQRPGSANGVIFMTIEDETGIANIIVWEKTFQKYRRQVM
GSRLVKVRGRLQNQSGVIHVVADHLEDITPMLGLLRREARRFGVNDRADGALRPSADARE
KKKLRQLRLGLPARAAPEGEAAAQVAEVMPKGRNFH