Protein Info for SMc03230 in Sinorhizobium meliloti 1021

Annotation: DNA-binding/iron metalloprotein/AP endonuclease

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 360 TIGR03723: tRNA threonylcarbamoyl adenosine modification protein TsaD" amino acids 3 to 324 (322 residues), 420.4 bits, see alignment E=4.2e-130 TIGR00329: metallohydrolase, glycoprotease/Kae1 family" amino acids 4 to 317 (314 residues), 329.6 bits, see alignment E=1.9e-102 PF00814: TsaD" amino acids 28 to 318 (291 residues), 319.5 bits, see alignment E=1.1e-99

Best Hits

Swiss-Prot: 100% identical to TSAD_RHIME: tRNA N6-adenosine threonylcarbamoyltransferase (tsaD) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K01409, O-sialoglycoprotein endopeptidase [EC: 3.4.24.57] (inferred from 100% identity to smk:Sinme_3131)

Predicted SEED Role

"TsaD/Kae1/Qri7 protein, required for threonylcarbamoyladenosine t(6)A37 formation in tRNA"

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.4.24.57

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q92LH8 at UniProt or InterPro

Protein Sequence (360 amino acids)

>SMc03230 DNA-binding/iron metalloprotein/AP endonuclease (Sinorhizobium meliloti 1021)
MRILGIETSCDETAASVVLRDEEGRGRILGDVVLSQLEEHSAYGGVVPEIAARAHVEALD
ALIEEALLRAGVTLRDIDAVAATSGPGLIGGLIVGLMTGKAIARATGKPLYAVNHLEGHA
LTARLTDGLSFPYLMLLVSGGHTQLILVKGVGEYERWGTTIDDALGEAFDKTAKLLGLPY
PGGPAVERAAQAGNAERFDFPRPLVGDARLDFSFSGLKTAVRQAAQSLGPVTDQDIADVC
ASFQRAISRTLRDRVGRGLKRFRADFASVDQPALVVAGGVAANQTLRRTLQSLCDEHGFR
FIAPPLQLCTDNAAMIAWAGAERLAAGLPADGLDAAPRSRWPLDSEAKALIGSGRRGAKA