Protein Info for SMc03112 in Sinorhizobium meliloti 1021
Annotation: B12-dependent methionine synthase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 61% identical to METH_ECOLI: Methionine synthase (metH) from Escherichia coli (strain K12)
KEGG orthology group: K00548, 5-methyltetrahydrofolate--homocysteine methyltransferase [EC: 2.1.1.13] (inferred from 80% identity to agr:AGROH133_07572)MetaCyc: 61% identical to cobalamin-dependent methionine synthase (Escherichia coli K-12 substr. MG1655)
Methionine synthase. [EC: 2.1.1.13]
Predicted SEED Role
"5-methyltetrahydrofolate--homocysteine methyltransferase (EC 2.1.1.13)" in subsystem Methionine Biosynthesis (EC 2.1.1.13)
MetaCyc Pathways
- aspartate superpathway (23/25 steps found)
- superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (16/18 steps found)
- superpathway of L-methionine biosynthesis (by sulfhydrylation) (11/12 steps found)
- folate transformations III (E. coli) (8/9 steps found)
- folate transformations II (plants) (9/11 steps found)
- superpathway of S-adenosyl-L-methionine biosynthesis (7/9 steps found)
- superpathway of L-methionine biosynthesis (transsulfuration) (7/9 steps found)
- superpathway of L-homoserine and L-methionine biosynthesis (6/8 steps found)
- L-methionine biosynthesis III (3/4 steps found)
- L-methionine salvage from L-homocysteine (2/3 steps found)
- folate transformations I (9/13 steps found)
- L-methionine biosynthesis I (3/5 steps found)
- superpathway of L-methionine salvage and degradation (9/16 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 2.1.1.13
Use Curated BLAST to search for 2.1.1.13
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See Q92LQ0 at UniProt or InterPro
Protein Sequence (1257 amino acids)
>SMc03112 B12-dependent methionine synthase (Sinorhizobium meliloti 1021) MSAADALFGNVSPKPDGSEVFRQLAQAAAERILIMDGAMGTEIQQLGFVEDHFRGERFGG CACHQQGNNDLLTLTQPKAIEDIHYHYAIAGADILETNTFSSTRIAQADYGMEDMVYDLN RDGARLARRAAKRAEAEDGRRRFVAGALGPTNRTASISPDVNNPGYRAVSFDDLRLAYAE QVRGLIDGGADIILIETIFDTLNAKAAIFATQEVFAEKGVRLPVMISGTITDLSGRTLSG QTPTAFWYSVRHADPFTIGLNCALGANAMRAHIDELSAVADTLVCAYPNAGLPNEFGRYD ESPEQMAAQVEGFARDGLVNIVGGCCGSTPAHIRAIAEAVAKYPPRRVPEIDRRMRLSGL EPFTLTDEIPFVNVGERTNVTGSAKFRKLITAGDYAAALDVARDQVANGAQIIDVNMDEG LIDSKQVMVEFLNLVASEPDIARVPVMIDSSKWEVIEAGLKCVQGKALVNSISLKEGEAA FLHHARLVRAYGAAVVVMAFDEKGQADTKTRKVEICRRAYRLLTEEVGFPPEDIIFDPNI FAVATGIEEHNNYGVDFIEATHEIIAALPHVHVSGGVSNLSFSFRGNEPVREAMHAIFLY HAIQAGMDMGIVNAGQLAVYDAIDPELRETCEDVVLNRRADSTERLLEIAERYRGKGGSQ GKEKDLAWREWPVEKRLEHALVNGITEFIEADTEEARLAAERPLHVIEGPLMAGMNVVGD LFGSGKMFLPQVVKSARVMKQAVAVLLPHMEEEKRANGGGEARESAGKILMATVKGDVHD IGKNIVGVVLACNNYEIIDLGVMVPSAKILEVAREQKVDIVGLSGLITPSLDEMAHVASE LEREGFDVPLLIGGATTSRVHTAVKINPRYSLGQTVYVTDASRAVGVVSSLLSPEVRDSY KKTVRAEYLKVADAHARNEAEKRRLPLSQARANAFRIDWDAHQPKVPSFLGTRVFEGWDL AELARYIDWTPFFQTWELKGVFPKILDDERQGAAARQLFEDAQAMVEKIVAEAWFAPKAV IGFWPAASMGDDVRLFADEVREAELATFFTLRQQMVKRDGRPNVALADFVAPAASGKRDY VGGFVVTAGIEEVAIAERFERANDDYSSIMVKALADRFAEAFAERMHEYVRKELWGYAPD EAFTPQELIAEPYAGIRPAPGYPAQPDHTEKETLFRLLDAEAAIGVRLTESYAMWPGSSV SGLYVGHPDSYYFGVAKIERDQVEDYADRKRMSVREVERWLSPILNYVPMPETEAAE