Protein Info for SMc03014 in Sinorhizobium meliloti 1021

Annotation: flagellar MS-ring protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 557 transmembrane" amino acids 21 to 43 (23 residues), see Phobius details amino acids 436 to 457 (22 residues), see Phobius details TIGR00206: flagellar M-ring protein FliF" amino acids 7 to 444 (438 residues), 218 bits, see alignment E=1.5e-68 PF01514: YscJ_FliF" amino acids 44 to 217 (174 residues), 182.5 bits, see alignment E=7.4e-58 PF08345: YscJ_FliF_C" amino acids 249 to 413 (165 residues), 123.1 bits, see alignment E=1.3e-39

Best Hits

Swiss-Prot: 100% identical to FLIF_RHIME: Flagellar M-ring protein (fliF) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K02409, flagellar M-ring protein FliF (inferred from 100% identity to smk:Sinme_0340)

Predicted SEED Role

"Flagellar M-ring protein FliF" in subsystem Flagellum

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See O54239 at UniProt or InterPro

Protein Sequence (557 amino acids)

>SMc03014 flagellar MS-ring protein (Sinorhizobium meliloti 1021)
MNLFDQFSTFTKNLSNLGQGKLIALAVAGVVAIGFVLGAGIYVNRPSFETLYVGLERSDV
TQISIALAEANVDFEVGTDGGSIQVPVGMTGKARLLLAERGLPSSANAGYELFDNVGSLG
LTSFMQEVTRVRALEGEIARTIQQISGIAAARVHIVMPERGSFRKAEQTPTASVMIRASA
TVGRSAASSIRHLVASSVPGLDVDDVTVLDSTGQLLASGDDPSNSALNQSLGVVQNVQSD
LEKKIDNALAPFLGMDNFRTSVTARLNTDAQQIQETVFDPESRVERSTRVIKEEQKSSQQ
QPDNAATVQQNVPQAAPRGGAGQQSSDEAEKKEEQTNYEINSKTIATVKNSYSIERLSIA
VVVNRGRLAAMAGEPADQAKIDAYLQEMQKIVSSAAGIDPGRGDVVTLNAMDFVETQLLD
QAVPGPGIMEMLTRNLGGIINALAFVAVAFLVVWFGMRPLARQLGFGGQAGKLEGEAAGL
ELPDFSPAGAGAGGALMEGFGSDFGFDGGDDLLNLGDEAGFNRRVKEGPERRLARMVEIS
EERAAKILRKWAVDRAA