Protein Info for SMc03010 in Sinorhizobium meliloti 1021

Annotation: chemotaxis-specific methylesterase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 349 PF00072: Response_reg" amino acids 7 to 110 (104 residues), 83 bits, see alignment E=1.7e-27 PF01339: CheB_methylest" amino acids 159 to 337 (179 residues), 213.3 bits, see alignment E=2.1e-67

Best Hits

Swiss-Prot: 100% identical to CHEB1_RHIME: Protein-glutamate methylesterase/protein-glutamine glutaminase of group 1 operon (cheB1) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K03412, two-component system, chemotaxis family, response regulator CheB [EC: 3.1.1.61] (inferred from 100% identity to smk:Sinme_0335)

Predicted SEED Role

"Chemotaxis response regulator protein-glutamate methylesterase CheB (EC 3.1.1.61)" in subsystem Bacterial Chemotaxis (EC 3.1.1.61)

Isozymes

Compare fitness of predicted isozymes for: 3.1.1.61

Use Curated BLAST to search for 3.1.1.61

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q52883 at UniProt or InterPro

Protein Sequence (349 amino acids)

>SMc03010 chemotaxis-specific methylesterase (Sinorhizobium meliloti 1021)
MSAPARVLVVDDSATMRGLISAVLNADPDITVVGQAADALEARQAIKQLDPDVVTLDIEM
PNMNGLEFLDKIMRLRPMPVIMVSTLTHRGAEATIAALEIGAFDCVGKPQPGDTHPFRDL
ADKVKAAARSQRKSMITSNRAAAPAATAVSDSRAGRKIVAIGASTGGVEALITVLQKFPA
NCPPTVITQHMPHTFTKSFAERLNRLCAPTVQEATDGARLEVGRVYLAPGGERHLEVHNA
AAPCCRLVDHPPVNGHRPSVDVLFDSVAELAGRNAIGVILTGMGRDGAAGLLKLRHAGAR
TFGQNEKTCVVYGMPRVAYELGAVETQLPLGSIGEEILKTAAARKEGIE