Protein Info for SMc03007 in Sinorhizobium meliloti 1021

Annotation: chemotaxis protein (sensory transduction histidine kinase)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 758 PF01627: Hpt" amino acids 5 to 95 (91 residues), 60.8 bits, see alignment E=2.5e-20 PF02895: H-kinase_dim" amino acids 360 to 421 (62 residues), 68.7 bits, see alignment 9.7e-23 PF02518: HATPase_c" amino acids 469 to 606 (138 residues), 70.3 bits, see alignment E=3.7e-23 PF01584: CheW" amino acids 612 to 742 (131 residues), 87.3 bits, see alignment E=1.5e-28

Best Hits

Swiss-Prot: 100% identical to CHEA_RHIME: Chemotaxis protein CheA (cheA) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K03407, two-component system, chemotaxis family, sensor kinase CheA [EC: 2.7.13.3] (inferred from 100% identity to smk:Sinme_0332)

Predicted SEED Role

"Signal transduction histidine kinase CheA (EC 2.7.3.-)" in subsystem Bacterial Chemotaxis or Flagellar motility or Two-component regulatory systems in Campylobacter (EC 2.7.3.-)

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3, 2.7.3.-

Use Curated BLAST to search for 2.7.13.3 or 2.7.3.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q52880 at UniProt or InterPro

Protein Sequence (758 amino acids)

>SMc03007 chemotaxis protein (sensory transduction histidine kinase) (Sinorhizobium meliloti 1021)
MDMNEIKEIFFQECEEQLAELESGLLKLNDGDRDPETVNAVFRAVHSIKGGAGAFGLDDL
VSFAHVFETTLDCVRSNRLEPNQDVLKVMLRSADVLADLTNAARDGGGVDEARSRQLIKE
LEALANGELPQAAAESAPKTTPAGVAPAAPVVNEEGFQPVAFSFDDFETGDEPTIEPSTY
EIVFKPKSDLYSKGNDATLLLRDLSRLGEMSIHCNMDTLPPLDRMNPEEAYFSWKISLKT
DKGEEAIRSVFEFAEWDCELDVALAGGTVGMDEDLPMQPVPFDLSILEDEAQAPAGEEDR
AAASEGDSRNAAVAAAQTASNVLQMAQSTARVSPENARNSQSASAAQAAAQQAASAATPT
IRVDLDRVDRLINLVGELVINQAMLSQSVIENDTNGTSSINMGLEELQQLTREIQDSVMA
IRAQPVKPVFQRMSRIVREIADMTGKSVRLITEGENTEVDKTVIDKLAEPLTHMIRNAVD
HGLETPEKRVAAGKNPEGTVRLTAKHRSGRIVIELADDGAGINREKVRQKAIDNDLIAAD
ANLSDEEVDNLIFHAGFSTADKISDISGRGVGMDVVKRSIQALGGRINISSKPGQGSIFT
MSLPLTLAVLDGMVVTVANQTLVVPLTAIVETLQPEASAIHSFGSSQRLISIRDSFCPLV
DVGRILNFRGAQANPVEGVALLVESEGGGQRALMVDAIQGQRQVVIKSLEANYTHVPGIA
AATILGDGRVALILDVDAIVAASRGQSLKPEMSLAAAG