Protein Info for SMc02914 in Sinorhizobium meliloti 1021

Annotation: translation initiation factor IF-2

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 889 PF08364: IF2_assoc" amino acids 15 to 51 (37 residues), 25.4 bits, see alignment (E = 5.2e-09) TIGR00487: translation initiation factor IF-2" amino acids 304 to 889 (586 residues), 794.7 bits, see alignment E=6.1e-243 PF04760: IF2_N" amino acids 311 to 362 (52 residues), 42.9 bits, see alignment 1.3e-14 TIGR00231: small GTP-binding protein domain" amino acids 391 to 545 (155 residues), 110.2 bits, see alignment E=8.8e-36 PF00009: GTP_EFTU" amino acids 391 to 547 (157 residues), 120.8 bits, see alignment E=2.2e-38 PF01926: MMR_HSR1" amino acids 392 to 497 (106 residues), 44.3 bits, see alignment E=7.1e-15 PF02421: FeoB_N" amino acids 392 to 546 (155 residues), 30.2 bits, see alignment E=1.2e-10 PF00071: Ras" amino acids 393 to 547 (155 residues), 25.3 bits, see alignment E=4e-09 PF11987: IF-2" amino acids 667 to 779 (113 residues), 145 bits, see alignment E=3.9e-46 PF03144: GTP_EFTU_D2" amino acids 810 to 877 (68 residues), 38.7 bits, see alignment 4.4e-13

Best Hits

Swiss-Prot: 100% identical to IF2_RHIME: Translation initiation factor IF-2 (infB) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K02519, translation initiation factor IF-2 (inferred from 80% identity to ara:Arad_0173)

Predicted SEED Role

"Translation initiation factor 2" in subsystem NusA-TFII Cluster or Translation initiation factors eukaryotic and archaeal or Universal GTPases

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q92SW4 at UniProt or InterPro

Protein Sequence (889 amino acids)

>SMc02914 translation initiation factor IF-2 (Sinorhizobium meliloti 1021)
MTDNKDDKTLSVAGKKTLTLKPSGVTQGTVRQDMGRGRTKAVVVETKRTRGPLKHKDERP
ITPVAATPAARPAEQRPMPPQPSGRPAPQPQPHQPRQEQNRPRGGVVLNDLSAGEMEARR
RALAEAQIRDAEEAKRRAEDEVRRRREEEERLAREKEEAARRAAEEAARPPVEAEKTEEK
VEAASPAVGERRAETRPQPGRAAPAATPAAPDGAALRGRRGTESEEDERRRSGAGAPRGK
VVRPEPAKPAPRAKGDEGRRQGKLTLTTAAVDEDGSQRGRSLSAMRRRQEKFKRSQMQET
REKISREVVLPETITIQELSQRMSERAVDVIKFLMKEGQMMKPGDLIDADLAELIAGEFG
HTVKRVSESDVEEGIFNISDVDDDMQSRPPIVTIMGHVDHGKTSLLDAIRHANVVAGEAG
GITQHIGAYQVEQNGQKITFIDTPGHAAFTAMRARGAQATDIAVLVVAADDSVMPQTIES
INHAKAAGVPIIVAINKIDKPSANPQKVRTELLQHEVFVESMGGEVLDVEVSAKNQTNLD
KLLEAILLQSEILDLKANPNRTAEGTVVEAELDRGRGAVATVLVQKGTLTPGQIIVAGDQ
WGRVRALVNDKGEHVKAAGPSTPVEVLGLSGTPAAGDRFAVVESESRAREISEYRQRLAR
EKAVARQSGSRGSLEQMMTQLQTSGVKEFPLVIKGDVQGSIEAISGALDKLGTDEVRARI
VHSGAGGITESDVSLAEASNAAIIGFNVRANKQARDASERAGIEIRYYNIIYDLVDDVKA
AMSGLLSPERRETFLGNAEILEVFNITKVGKVAGCRVTEGKVERGVGVRLVRDNVVIHEG
KLKTLKRFKDEVSEVQSGQECGMAFENYEDIRAGDTIECFRVEHVTRTL