Protein Info for SMc02858 in Sinorhizobium meliloti 1021

Annotation: chaperone protein DnaJ

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 379 PF00226: DnaJ" amino acids 4 to 66 (63 residues), 96.1 bits, see alignment E=1.6e-31 TIGR02349: chaperone protein DnaJ" amino acids 4 to 353 (350 residues), 443.9 bits, see alignment E=2.5e-137 PF01556: DnaJ_C" amino acids 123 to 337 (215 residues), 183.4 bits, see alignment E=4.3e-58 PF00684: DnaJ_CXXCXGXG" amino acids 150 to 210 (61 residues), 58.4 bits, see alignment E=1e-19

Best Hits

Swiss-Prot: 100% identical to DNAJ_RHIME: Chaperone protein DnaJ (dnaJ) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K03686, molecular chaperone DnaJ (inferred from 100% identity to smk:Sinme_3510)

MetaCyc: 56% identical to chaperone protein DnaJ (Escherichia coli K-12 substr. MG1655)
1.8.4.-

Predicted SEED Role

"Chaperone protein DnaJ" in subsystem Heat shock dnaK gene cluster extended or Protein chaperones

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q92T07 at UniProt or InterPro

Protein Sequence (379 amino acids)

>SMc02858 chaperone protein DnaJ (Sinorhizobium meliloti 1021)
MKRDLYETLGVQKNADEKELKSAFRKLAMKYHPDRNPGDQESEKSFKEINEAYETLKDPQ
KRAAYDRYGHAAFEQGGMGAGFGNGFAGGGAHGFSDIFEDIFGEMMGGRQRRSSGGRERG
ADLRYNMEISLEEAYSGKTAQIRVPTSITCDVCTGTGAKPGTSPKTCGTCQGTGRVRAAQ
GFFSIERTCPTCGGRGQTIADPCTKCHGQGRVVEERTLSVNIPAGIEDGTRIRLSGEGEA
GLRGGPAGDLYIFLSVKPHEFYQRDGADLYCAVPISMTTAALGGKFDVTTLDGTKSRVTV
PEGTQAGKQFRLKGKGMPVLRSSQTGDLYIQIQIETPQKLTKRQRELLQEFEQISSKENN
PESTGFFSRMKDFFDTLSE