Protein Info for SMc02850 in Sinorhizobium meliloti 1021

Annotation: DNA polymerase I

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1004 TIGR00593: DNA polymerase I" amino acids 7 to 1004 (998 residues), 925.5 bits, see alignment E=2.2e-282 PF02739: 5_3_exonuc_N" amino acids 7 to 176 (170 residues), 186 bits, see alignment E=8.7e-59 PF01367: 5_3_exonuc" amino acids 177 to 271 (95 residues), 111.5 bits, see alignment E=4.7e-36 PF01612: DNA_pol_A_exo1" amino acids 386 to 588 (203 residues), 91.9 bits, see alignment E=8.4e-30 PF00476: DNA_pol_A" amino acids 621 to 1002 (382 residues), 523.5 bits, see alignment E=6.2e-161

Best Hits

KEGG orthology group: K02335, DNA polymerase I [EC: 2.7.7.7] (inferred from 77% identity to avi:Avi_0299)

Predicted SEED Role

"DNA polymerase I (EC 2.7.7.7)" in subsystem DNA-replication or DNA Repair Base Excision (EC 2.7.7.7)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.7

Use Curated BLAST to search for 2.7.7.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q92T14 at UniProt or InterPro

Protein Sequence (1004 amino acids)

>SMc02850 DNA polymerase I (Sinorhizobium meliloti 1021)
MKNGDHLFLVDGSGFIFRAFHAIPPLNRKSDGLPVNAVAGFCNMLWKLLTDARDTSVGVT
PTHLAVIFDYSSKTFRNGLYDQYKANRTAPPEDLIPQFGLIRHATRAFNLPCIEKEGYEA
DDLIATYARLAEEAGADVTIVSSDKDLMQLVTPKVSMYDSMKDKQITVPDVIEKWGVPPE
KMIDLQAMTGDSTDNVPGIPGIGPKTAAQLLEEYGDLDTLLARAGEIKQQKRRESIIANA
DLARLSRELVTLKKDTPLDVPPEDFRLDSQDGPKLIAFLKAMEFTTLTRRVAAATDTDAE
AIEPAHVPVEWGAQAHGPDLDVGEAGGPPPSPQSSSATPPRGNAARAAVSFLSSGQDADT
TGATPTGLAEARAAYFGKAPFDHSGYRTIRDIDTLERWIADAREAGLVGFDTQATSPDAM
RADLVGFSLAVADYANDPSGSRIRAAYVPLAHKSGVSDLLGGGPVDSQVPGRETLSRLKE
LLEDPSVLKVGQNLKYGYLVMKRHGIAMRSFDDTMLMSYVLDAGNGAHGMDSLAERWLGH
TPIAYKDVTGTGRSSLTFDFVDIDKATAYAAEDADIALRLWHVLKPRLAAKGLTRVYERL
ERPLISVLAGMEERGITVDRQILSRLSGELAQGAAALEDEIYRLAGETFTIGSPKQLGDI
LFGKMGLPGGSKTKTGQWSTSAQVLEDLAAAGHDLPRKIVDWRQLTKLKSTYTDALPGFV
HPETKRVHTCFAMAATTTGRLSSSDPNLQNIPIRTGEGRKIRTAFVATPGHKLVSADYSQ
IELRVLAHVADIPQLRQAFADGVDIHAMTASEMFGVPVDGMPSEIRRRAKAINFGIIYGI
SAFGLANQLSIERSEAGDYIKRYFERFPGIRDYMENTKAFARENGYVETIFGRRAHYPDI
RSSNPSMRAFNERASINAPIQGSAADIIRRAMVKMEPALEAAKLSARMLLQVHDELIFEV
EDGEIERTIPVIISVMENAAMPALDMRVPLKVDARAAHNWDEAH