Protein Info for SMc02717 in Sinorhizobium meliloti 1021

Annotation: 2-isopropylmalate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 569 TIGR00970: 2-isopropylmalate synthase" amino acids 17 to 563 (547 residues), 757.2 bits, see alignment E=5.3e-232 PF00682: HMGL-like" amino acids 41 to 318 (278 residues), 249.2 bits, see alignment E=5.4e-78 PF08502: LeuA_dimer" amino acids 433 to 564 (132 residues), 101.3 bits, see alignment E=4e-33

Best Hits

Swiss-Prot: 100% identical to LEU1_RHIME: 2-isopropylmalate synthase (leuA) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K01649, 2-isopropylmalate synthase [EC: 2.3.3.13] (inferred from 100% identity to sme:SMc02717)

Predicted SEED Role

"2-isopropylmalate synthase (EC 2.3.3.13)" in subsystem Branched-Chain Amino Acid Biosynthesis or Leucine Biosynthesis (EC 2.3.3.13)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.3.3.13

Use Curated BLAST to search for 2.3.3.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q9X7L2 at UniProt or InterPro

Protein Sequence (569 amino acids)

>SMc02717 2-isopropylmalate synthase (Sinorhizobium meliloti 1021)
MILKSHSIKTGMPEAAVKYQPYPQIVLPDRTWPSKAITEAPIWCSVDLRDGNQALVDPMG
HDRKARMFHLLLDMGFKEIEIGFPSASQTDYDFARWCVEEGNVSEDVSLQVLVQCRPELI
ARTFEALEGAHRPIVHFYNSTSELQRRVVFGKDVAGIKQIATDAAKMITDMAAKAGGGYR
FEYSPESFTGTELEVALEICNAVIEIVRPTADNKLIINLPSTVEMATPNIYADQIEWMCR
NLDNRENLIVSLHPHNDRGTGIAATELGLMAGADRVEGTLFGNGERTGNVDVVTLALNMF
TQGVDPKLDCSDIERIKEVYEYSNQMVIPERHPYVGELVYTAFSGSHQDAINKGMKAIKQ
ANKPTWEVPYLPIDPRDVGRSYEAIIRINSQSGKGGIAYILQEDYGINLPRNLQIEFREE
VQRITDEEGKELPSKRIHQRFIESYVEQPGARIKFVDHHTYPAGEHKGLRVVAAEITDGG
ETRQIEGKGTGPIDGFINALSIYLGIELSVADYSEHSLQHGSNAAAIAYVEVEYPGGKLF
GVGINTNIVAASLEAIVSAANRVLDVVGK