Protein Info for SMc02700 in Sinorhizobium meliloti 1021

Annotation: hypothetical protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 419 PF01743: PolyA_pol" amino acids 30 to 151 (122 residues), 90.7 bits, see alignment E=1e-29 PF12627: PolyA_pol_RNAbd" amino acids 187 to 239 (53 residues), 36.3 bits, see alignment 4e-13

Best Hits

KEGG orthology group: K00970, poly(A) polymerase [EC: 2.7.7.19] (inferred from 100% identity to sme:SMc02700)

Predicted SEED Role

"tRNA nucleotidyltransferase (EC 2.7.7.21) (EC 2.7.7.25)" in subsystem Polyadenylation bacterial or tRNA nucleotidyltransferase (EC 2.7.7.21, EC 2.7.7.25)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.7.19 or 2.7.7.21 or 2.7.7.25

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q92N60 at UniProt or InterPro

Protein Sequence (419 amino acids)

>SMc02700 hypothetical protein (Sinorhizobium meliloti 1021)
MTSIVAERWFQAPHLRRIFDLLNADGGEVRVVGGAIRNSLMGLPAGDVDLATTWRPEEVT
ERAGGAGIKAVPTGIDHGTVTLVIEGVPYEITTLRRDVATDGRRAEVAFGTDWKADAERR
DFTINALYAGVDGEIIDHVGGLGDIETRTVRFIGSAAERVAEDYLRILRFFRFFAHYGSG
RPDAEGLRACAQARAKLATLSAERVWTETKKLLSADDPGRALLWMRQAGVLSEILPETEK
WGIDAIPALIAAEKSFSWRADPLLRLAAIVPPDAARLEAMTKRLRLSKAESAYLDRFAEA
PEIAATLADAALERQLYRHGTEGIVVRLRLALASARRKAETDPAFLADSASFHRLLARAE
KWQRPVFPLNGADVLKLGIPAGPKVGEVLAELEAFWVERNFSVERATLVARLESMVRSG