Protein Info for SMc02686 in Sinorhizobium meliloti 1021

Annotation: ribose-phosphate pyrophosphokinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 310 PF13793: Pribosyltran_N" amino acids 1 to 117 (117 residues), 180.4 bits, see alignment E=1.5e-57 TIGR01251: ribose-phosphate diphosphokinase" amino acids 1 to 310 (310 residues), 392 bits, see alignment E=7.6e-122 PF00156: Pribosyltran" amino acids 148 to 252 (105 residues), 85.1 bits, see alignment E=4.9e-28 PF14572: Pribosyl_synth" amino acids 199 to 309 (111 residues), 78.4 bits, see alignment E=1.1e-25

Best Hits

Swiss-Prot: 100% identical to KPRS_RHIME: Ribose-phosphate pyrophosphokinase (prs) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K00948, ribose-phosphate pyrophosphokinase [EC: 2.7.6.1] (inferred from 100% identity to smd:Smed_2257)

MetaCyc: 55% identical to ribose-phosphate diphosphokinase (Escherichia coli K-12 substr. MG1655)
Ribose-phosphate diphosphokinase. [EC: 2.7.6.1]

Predicted SEED Role

"Ribose-phosphate pyrophosphokinase (EC 2.7.6.1)" in subsystem De Novo Purine Biosynthesis or Pentose phosphate pathway (EC 2.7.6.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.6.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q92N73 at UniProt or InterPro

Protein Sequence (310 amino acids)

>SMc02686 ribose-phosphate pyrophosphokinase (Sinorhizobium meliloti 1021)
MKVFAGNSNRLLAEAICNYLNLPLGKATVRRFADQEIFVEIGENVRGEDVFIVQSTSFPT
NDHLMELLIMIDAVRRSSARRITAVLPYFGYARQDRKPGPRTPISAKLVANLITEAGADR
VLTLDLHAGQIQGFFDIPTDNLYAIPILARDVKENYNLKNVMVVSPDVGGVVRARALAKR
LDCLLAIVDKRRDRPGESEVMNVIGEVNGKDCLLIDDIVDSGGTLCNAAEALLKNGATSV
TAYITHGVLSGGAVARVTSSMLKELVITDSIQPTTAVQSAHNIRVISTAALLGEAISRTS
QEESVSSLFD