Protein Info for SMc02659 in Sinorhizobium meliloti 1021

Annotation: GTP pyrophosphokinase (ATP:GTP 3'-pyrophosphotransferase) protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 741 PF13328: HD_4" amino acids 26 to 175 (150 residues), 166.9 bits, see alignment E=1e-52 TIGR00691: RelA/SpoT family protein" amino acids 26 to 737 (712 residues), 658.1 bits, see alignment E=8e-202 PF01966: HD" amino acids 45 to 144 (100 residues), 35.9 bits, see alignment E=2.9e-12 PF04607: RelA_SpoT" amino acids 236 to 345 (110 residues), 139.1 bits, see alignment E=2.5e-44 PF02824: TGS" amino acids 394 to 451 (58 residues), 73.3 bits, see alignment 4.7e-24 PF19296: RelA_AH_RIS" amino acids 463 to 649 (187 residues), 270.8 bits, see alignment E=2.7e-84 PF13291: ACT_4" amino acids 661 to 738 (78 residues), 61.7 bits, see alignment E=3e-20

Best Hits

Swiss-Prot: 71% identical to RSH_BRUAB: GTP pyrophosphokinase rsh (rsh) from Brucella abortus biovar 1 (strain 9-941)

KEGG orthology group: K01139, guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase [EC: 3.1.7.2] (inferred from 100% identity to sme:SMc02659)

Predicted SEED Role

"GTP pyrophosphokinase (EC 2.7.6.5), (p)ppGpp synthetase II / Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (EC 3.1.7.2)" in subsystem Stringent Response, (p)ppGpp metabolism (EC 2.7.6.5, EC 3.1.7.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.6.5 or 3.1.7.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q7APE1 at UniProt or InterPro

Protein Sequence (741 amino acids)

>SMc02659 GTP pyrophosphokinase (ATP:GTP 3'-pyrophosphotransferase) protein (Sinorhizobium meliloti 1021)
MMRQYELVERVQKYKPDVNEALLNKAYVYAMQKHGQQKRASGDPYISHPLEVAAILTEMH
LDESTIAVALLHDTIEDTTATRQEIDDLFGEDIGALVEGLTKIKKLDLVTKKAKQAENLR
KLLLAISDDVRVLLVKLADRLHNMRTLDHMSAEKRARISEETMDIYAPLAGRMGMQDMRE
ELENLSFRHINPEAYETVTRRLQELSERNEGLIKKIEEELSELLQAEGLTDAQVRGRQKK
PYSVFRKMQSKSLSFEQLSDVWGFRIIVNDIPSCYRALGIVHTRWRVVPGRFKDYVSTPK
QNDYQSIHTTIIGPSRQRIELQIRTKRMHEIAEYGIAAHALYKDRDTVSGDLTRTPTSNA
YSWLRRTIESLAEGDNPEEFLEHTKLELFQDQVFCFTPKGQLIALPRGATPIDFAYAVHT
NIGDTCVGAKINGRIMPLVTRLNNGDEVEIIRSGIQVPPPAWEEIVVTGKARAAIRRATR
AAIRKQYAGLGYRILERTFERAGKTFSREALKPVLHRLGQKDVEDAIAAVGRGELSSLDV
LRAVFPDHQDERVTVKPSADDGWFNMRSAAGMIFKLPERSKEMAAAEQAEGPEALPIRGL
SGNAEVHFSPAGAVPGDRIVGIMDKDKGITIYPIQSPILQKFDEEPERWIDVRWDLDEAN
NSRFMARIAVSALNEPGTLAEVAQAIATTDVNIRSLSMGRVAADFSELQFDLEVWDLRQL
NHLMAQLKELPSISMVKRLFE