Protein Info for SMc02606 in Sinorhizobium meliloti 1021

Annotation: sarcosine oxidase subunit alpha

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 987 transmembrane" amino acids 105 to 126 (22 residues), see Phobius details TIGR01372: sarcosine oxidase, alpha subunit family" amino acids 3 to 987 (985 residues), 1481.1 bits, see alignment E=0 PF13510: Fer2_4" amino acids 17 to 100 (84 residues), 90.4 bits, see alignment E=2.6e-29 PF00890: FAD_binding_2" amino acids 170 to 206 (37 residues), 22.3 bits, see alignment (E = 2.9e-08) PF12831: FAD_oxidored" amino acids 170 to 218 (49 residues), 36.9 bits, see alignment 1.2e-12 PF07992: Pyr_redox_2" amino acids 170 to 359 (190 residues), 40.8 bits, see alignment E=7.7e-14 PF13450: NAD_binding_8" amino acids 173 to 215 (43 residues), 24.1 bits, see alignment (E = 1.5e-08) PF17806: SO_alpha_A3" amino acids 501 to 584 (84 residues), 103.1 bits, see alignment E=3.4e-33 PF01571: GCV_T" amino acids 600 to 871 (272 residues), 283 bits, see alignment E=7.4e-88 PF08669: GCV_T_C" amino acids 893 to 979 (87 residues), 52.6 bits, see alignment E=1.4e-17

Best Hits

Swiss-Prot: 100% identical to SOXA_RHIME: Sarcosine oxidase subunit alpha (soxA) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K00302, sarcosine oxidase, subunit alpha [EC: 1.5.3.1] (inferred from 100% identity to sme:SMc02606)

Predicted SEED Role

"Sarcosine oxidase alpha subunit (EC 1.5.3.1)" in subsystem Choline and Betaine Uptake and Betaine Biosynthesis (EC 1.5.3.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.5.3.1

Use Curated BLAST to search for 1.5.3.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See O87386 at UniProt or InterPro

Protein Sequence (987 amino acids)

>SMc02606 sarcosine oxidase subunit alpha (Sinorhizobium meliloti 1021)
MSSYRLPKRGLVDRNVPLSFTFDGRPMQGLEGDTLASALLANGRMLVGRSFKYHRPRGIL
TAGAAEPNALVTVGRGGRAEPNTRATMQELYEGLEARSQNRWPSLAFDIGALNGLLSPFL
GAGFYYKTFMWPAPLWEKLYEPVIRRAAGLGKASYEADPDAYEKSWAHCDLLVIGAGPTG
LAAALTAGRAGARVILVDEGSLPGGSLLSDTATIDGKAAADFARDTSDELRSMPNVQVLV
RTTAFGWYDGNVFGAVERVQKHVREPASHLPVERLWRIVAGKALLATGAEERPLVFGGND
RPGVMMAGAMRAYLNRYGVAPGRTPAIFTTNDTGYTLAQELEAAGVDVVAIVDSRPAAGV
DYRGKARLVREAVVCGTKGGKAISAIEVHHGGRTETIAVDALAMAGGFDPIIHLACHRGG
KPVWSAEKAAFLAPGSLKGLEVAGGAAATTGLAACLGEGAARAEAIVRELGLPCPPVAVV
KVESEEGIRSPAPLWSIPGIKDKAFVDFQNDVHLKDIGLAVREGYSHVELAKRYTTSGMA
TDQGKLSNVNAIGLIAKARGVSPAEVGTTTFRPFYTPVSFGALTGAHTGHHFQPVRKSPL
HDWAKKHGAVFVETGLWYRSSWFPRSGERTWRESVEREVLNVRKNAGLCDVSMLGKIEIT
GSDAAEFLNRVYCNAFLKLPVGKARYGLMLREDGFIYDDGTTSRLEENRFFMTTTTAYAA
GVMNHLEFCAQVLWPQLDVRLASITDQWAQMAIAGPKARMILQKIVDEDISDAAFPFLAA
KEVSLFGGALHGCLFRISFSGELAYELAVPAGYGESIADALLEAGKDHGIMPYGVETLSV
LRIEKGHVTHNEINGTIVPADLGFGKMVSAGKPDFVGKAMLQREGLTAPDRPQLVGVVPL
DPQQSFRSGSHILAKGAAATLENDEGYVTSSAYSPHVGSTIALALVRNGRNRHGEEVLVW
SGLHGESTPARLCNPVFFDPQNERLHV