Protein Info for SMc02586 in Sinorhizobium meliloti 1021

Annotation: ATP-dependent helicase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 820 TIGR01970: ATP-dependent helicase HrpB" amino acids 7 to 819 (813 residues), 1015.7 bits, see alignment E=0 PF00270: DEAD" amino acids 22 to 166 (145 residues), 43 bits, see alignment E=6.3e-15 PF00271: Helicase_C" amino acids 208 to 331 (124 residues), 51.2 bits, see alignment E=2.1e-17 PF08482: HrpB_C" amino acids 675 to 806 (132 residues), 156.1 bits, see alignment E=9.6e-50

Best Hits

KEGG orthology group: K03579, ATP-dependent helicase HrpB [EC: 3.6.4.13] (inferred from 100% identity to sme:SMc02586)

Predicted SEED Role

"macromolecule metabolism; macromolecule synthesis, modification; dna - replication, repair, restr./modif."

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.13

Use Curated BLAST to search for 3.6.4.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q92TA0 at UniProt or InterPro

Protein Sequence (820 amino acids)

>SMc02586 ATP-dependent helicase (Sinorhizobium meliloti 1021)
MRALPALPIREILPGLGDALASAGSVVLSAPPGAGKTTLVPLFLLDQPWRGDGKIILLEP
RRLAARAAAGRMAELLGEKVGETVGYRMRLDNRVSGKTRIEVVTEGVFSRMILDDPELSG
VSAVLFDEFHERSLDADFGLALALDVQSALREDLRIVVMSATLDVERIAGLVGDAPVLKS
EGRSFPIDIRYENRAAGESVEDAMVRTIAEAHRSEKGSILAFLPGQAEIARTAARLADRF
GEATAIVPLYGNLSQKEQDAAIRPAPKGTRKIVLATSIAETSITIDGVRIVVDSGLQRLP
VFEASTGITRLETVRVSRASADQRAGRAGRTEPGIAIRLWHSGQTAALAAFTPPQILASD
LSGLLLDLAHWGVADPSTLRFLDPPPETTLREARGLLLELGAIDSRGALTPRGRRIRDLA
LPVRLAAMAVAAAEEGRAQEACLLAVMLTEQGLGGNGIDIEERLRRFRSERSDRAKAARG
LARRMAAELGASKNAGPKPVLPGALLMHAFPDRIALQRGGRGRFVMANGRGAEIPETERL
AAAGMLVIADLTGRAGAQRVLAAAEIDRSDVEGHMPEAIVTEEQSFFDRASRQVRARRVT
RLGAIIFEEKPLPRPSGEAAARALADGIRQLGLAAVPFPKDVEQLRDRIGFLHRSIGEPW
PDMSDTGLISRLDEWFVPFQGGAGGIDGIKGRDLAEGLMSLVPYELQRDLARLAPTHFEA
PTGQRHPIHYDGDEPLLSIRVQELFGLKTHPAIGDGRLPLLLELISPGHRPIQTTRDLPG
FWAGSWKDVRAEMRGRYPKHPWPEDPANAMPTTRAKPRGT