Protein Info for SMc02546 in Sinorhizobium meliloti 1021

Annotation: alpha-isopropylmalate/homocitrate synthase family transferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 538 TIGR00977: citramalate synthase" amino acids 6 to 530 (525 residues), 610.4 bits, see alignment E=1.1e-187 PF00682: HMGL-like" amino acids 6 to 276 (271 residues), 179.5 bits, see alignment E=9.8e-57 PF08502: LeuA_dimer" amino acids 381 to 523 (143 residues), 93.6 bits, see alignment E=1e-30

Best Hits

KEGG orthology group: K01649, 2-isopropylmalate synthase [EC: 2.3.3.13] (inferred from 100% identity to sme:SMc02546)

Predicted SEED Role

"(R)-citramalate synthase (EC 2.3.1.182)" in subsystem Branched-Chain Amino Acid Biosynthesis (EC 2.3.1.182)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.3.3.13

Use Curated BLAST to search for 2.3.1.182 or 2.3.3.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q92R15 at UniProt or InterPro

Protein Sequence (538 amino acids)

>SMc02546 alpha-isopropylmalate/homocitrate synthase family transferase (Sinorhizobium meliloti 1021)
MTRERIYLFDTTLRDGQQTPGIDFSVEDKIAIASLLDQFGMDYVEGGYPGANPTDTEFFK
CRRTDKASFVAFGMTKRAGVSASNDPGLSALLSAKSDAICLVAKSWDYHVEVALGCTNEE
NLDNIRASVEAVVASGREAMVDCEHFFDGYKANSAYALACAKTAFEAGARWVVLCDTNGG
TQPPEIREIVSAVIAEGVPGANLGIHAHNDTGQAVANSLAAVEAGVRQIQGTLNGIGERC
GNANLVTLIATLALKETYSSRFETGIDAERLQELTKLAHAFDELLNRSPDPQAPYVGASA
FATKAGIHASALLKDPRTYEHVAPESVGNLRKVMVSDQGGKANFINALKRRGITVSKDDP
RLDKLISIVKEREATGYAYEGADASFALLASRILGTVPDFFRVESFRVMVERRFDANGKL
KTVSEAVVRVIVDGETMMSVAEGHGPVNALDLALRKDLGKYQSEIADLELADYKVRILNG
GTEAVTRVLIESTDRSGARWWTVGVSDNIIDASFQALMDSIVYKLLKNRDQVGSIAAE