Protein Info for SMc02437 in Sinorhizobium meliloti 1021

Annotation: phosphoenolpyruvate phosphotransferase PTSP (enzyme I-Ntr) protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 755 PF13185: GAF_2" amino acids 24 to 159 (136 residues), 56.9 bits, see alignment E=6.9e-19 PF01590: GAF" amino acids 25 to 157 (133 residues), 73.9 bits, see alignment E=4.8e-24 TIGR01417: phosphoenolpyruvate-protein phosphotransferase" amino acids 183 to 741 (559 residues), 416.5 bits, see alignment E=8.1e-129 PF05524: PEP-utilisers_N" amino acids 185 to 303 (119 residues), 78.2 bits, see alignment E=1.5e-25 PF00391: PEP-utilizers" amino acids 330 to 401 (72 residues), 58.8 bits, see alignment E=8.4e-20 PF02896: PEP-utilizers_C" amino acids 430 to 716 (287 residues), 302.1 bits, see alignment E=1e-93

Best Hits

KEGG orthology group: K08484, phosphotransferase system, enzyme I, PtsP [EC: 2.7.3.9] (inferred from 100% identity to smk:Sinme_2667)

Predicted SEED Role

"Phosphoenolpyruvate-protein phosphotransferase, nitrogen regulation associated"

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.3.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q92MK4 at UniProt or InterPro

Protein Sequence (755 amino acids)

>SMc02437 phosphoenolpyruvate phosphotransferase PTSP (enzyme I-Ntr) protein (Sinorhizobium meliloti 1021)
MRDLSAGPRVLLKRLRELMAEPLEPQERLDRIVRQIAQNMVAEVCSVYVLRSDGVLELYA
TEGLNKTAVHLAQLKMGQGLVGTIAASARPLNLSDAQSHPAFTYLPETGEEIYHSFLGVP
ILRTGRALGVLVVQNKAQRNYREDEVEALETTAMVLAEMVATGELKKITKPGLELDLSRP
VTIEGNSYGEGIGLGYVVLHEPRIVVTNLLNEDTEHELQRLAEALGSLRISIDDMLSRRD
VSMEGEHRAVLETYRMFAHDRGWVRKLEEAIRNGLTAEAAVERVQSETKARMIRLTDPYL
RERMHDFDDLANRLLRQLSGYGAKLSAADFPNDAIVVARAMGAAELLDYPRENVRGLVLE
EGAVTSHVVIVARAMGIPVVGQAAGAVALAENRDAIIVDGDDAKVHLRPMADLQRAYEEK
VRFRARRQAQFRALRDVEPLTKDGKRITLQMNAGLLVDLPHLNEAGAEGIGLFRTELQFM
IASTMPKAEEQEAFYRNVMKQTAGKPVTFRTLDIGGDKVVPYFRAAEEENPALGWRAIRL
SLDRPGLLRTQFRAMLRATAGAELKMMLPMVTEVAELKSARELLQKEIERQSKLGEQLPR
KLQFGAMLEVPALLWQLDELMAEVDFVSVGSNDLFQFAMAVDRGNARVSERFDILGRPFL
RILREIVRAGERNNTPVTLCGEMASKPLSAMALLGLGFRSVSMSPTAVGPVKAMLLALDA
GRLNELLEAALDDVKSDTSIRQLLVDFAAANGIPV