Protein Info for SMc02433 in Sinorhizobium meliloti 1021

Annotation: ATP-dependent protease

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 868 TIGR03346: ATP-dependent chaperone protein ClpB" amino acids 7 to 858 (852 residues), 1408.9 bits, see alignment E=0 PF02861: Clp_N" amino acids 17 to 60 (44 residues), 47.2 bits, see alignment 1.2e-15 amino acids 94 to 145 (52 residues), 46.9 bits, see alignment 1.5e-15 PF00004: AAA" amino acids 204 to 316 (113 residues), 42.4 bits, see alignment E=5.6e-14 amino acids 604 to 722 (119 residues), 32.4 bits, see alignment E=7.2e-11 PF17871: AAA_lid_9" amino acids 343 to 445 (103 residues), 126.3 bits, see alignment E=2.6e-40 PF07724: AAA_2" amino acids 598 to 762 (165 residues), 235.8 bits, see alignment E=1.6e-73 PF07728: AAA_5" amino acids 603 to 724 (122 residues), 51.1 bits, see alignment E=8.6e-17 PF10431: ClpB_D2-small" amino acids 768 to 848 (81 residues), 99.2 bits, see alignment E=6.7e-32

Best Hits

Swiss-Prot: 100% identical to CLPB_RHIME: Chaperone protein ClpB (clpB) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K03695, ATP-dependent Clp protease ATP-binding subunit ClpB (inferred from 88% identity to ara:Arad_4124)

MetaCyc: 61% identical to protein disaggregase ClpB (Escherichia coli K-12 substr. MG1655)
RXN185E-10

Predicted SEED Role

"ClpB protein" in subsystem Protein chaperones or Proteolysis in bacteria, ATP-dependent

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q92MK7 at UniProt or InterPro

Protein Sequence (868 amino acids)

>SMc02433 ATP-dependent protease (Sinorhizobium meliloti 1021)
MDIEKYSERVRGFLQSAQTYALAEGHQQFTPEHVLKVLLDDDQGMAASLIERAGGNAREA
KVGTAAALAKLPKVTGGNGSVYLSQPLAKVFTAAEDAAKKAGDSFVTVERLLLALAIESS
AATASILSKAGVTPTKLNQVINEIRKGRTADSANAEQGFDSLKKYARDLTAEAREGKLDP
VIGRDDEIRRTIQVLSRRTKNNPVLIGEPGVGKTAIAEGLALRIVNGDVPESLKDKRLMA
LDMGALIAGAKFRGEFEERLKAVLNEVRSEGGEIILFIDEMHTLVGAGKADGAMDASNLL
KPALARGELHCVGATTLDEYRKHVEKDAALARRFQPVMVEEPTVEDTISILRGLKEKYEQ
HHKVRISDSALVAAAALSNRYITDRFLPDKAIDLMDEAASRLRMQVDSKPEELDELDRRV
IQLKIEREALKKETDVSSKDRLAKLELDLSSLEEEAAALTARWQAEKQKLGQAADLKKQL
DEARNELQIAQRKGEFQRAGELAYGVIPNLEKELAEAESQDGASANPMVQEVVTPDNIAH
IVSRWTGIPVDKMLEGERDKLLRMEDELAKWVVGQGDAVQAVSRAVRRSRAGLQDPNRPI
GSFIFLGPTGVGKTELTKALARFLFDDETALMRIDMSEYMEKHSVARLIGAPPGYVGYEE
GGALTESVRRRPYQVVLFDEIEKAHPDVFNVLLQVLDDGRLTDGQGRTVDFKNTMIIMTS
NLGAEYLTALGENEDSDAVRDQVMEVVRAAFRPEFLNRVDEIILFHRLRRSEMGAIVDIQ
LERLRKLLSDRKITLELEDDARVFLADRGYDPAYGARPLKRAIQKYVQDPLAEKVLQGEF
PDGSVIKVVAGSDRLNFKRGAGANKEAA