Protein Info for SMc02236 in Sinorhizobium meliloti 1021

Updated annotation (from data): DNA repair nuclease, ligase-associated (TIGR04123)
Rationale: Specifically important in stress Cisplatin. This family was previously annotated as a DNA ligase-associated metallophosphoesterase (TIGR04123). A member of this family (PdeM) has been studied biochemically and found to have nuclease activity, but it was not known if DNA was the physiological substrate (see PMC4320098).
Original annotation: hypothetical protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 242 TIGR04123: metallophosphoesterase, DNA ligase-associated" amino acids 23 to 232 (210 residues), 221.2 bits, see alignment E=4.8e-70 PF00149: Metallophos" amino acids 43 to 157 (115 residues), 33.1 bits, see alignment E=4.1e-12

Best Hits

KEGG orthology group: K06953, (no description) (inferred from 100% identity to sme:SMc02236)

Predicted SEED Role

"FIG006285: ICC-like protein phosphoesterase"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q92S83 at UniProt or InterPro

Protein Sequence (242 amino acids)

>SMc02236 DNA repair  nuclease, ligase-associated (TIGR04123) (Sinorhizobium meliloti 1021)
MTMHRLIYATSPAVNPSLHARTSINGVAAICDPLGGLYFPESRTLVVSDLHLEKGSAFAR
RGMMLPPYDTLATLRILDAVIARHEPATVISLGDNFHDRKGSAAMPETFRQMIAAMACGR
EWIWINGNHDPDGAQGLPGASMDELRHAGLVFRHEPSRRDGIGEIAGHLHPSATVRRRER
SVRRACFATDGKRLVMPAFGVTTGGLDLRHREMSGLFDRQQLVAHMLGRDRIYSVRFANL
LG