Protein Info for SMc02062 in Sinorhizobium meliloti 1021

Annotation: protein-L-isoaspartate O-methyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 204 PF01135: PCMT" amino acids 5 to 198 (194 residues), 131.8 bits, see alignment E=8e-42 PF05175: MTS" amino acids 58 to 139 (82 residues), 28.4 bits, see alignment E=2.9e-10 PF13847: Methyltransf_31" amino acids 66 to 147 (82 residues), 31.3 bits, see alignment E=4e-11 PF13649: Methyltransf_25" amino acids 71 to 147 (77 residues), 34.2 bits, see alignment E=8.3e-12 PF08241: Methyltransf_11" amino acids 72 to 147 (76 residues), 30.2 bits, see alignment E=1.4e-10

Best Hits

Swiss-Prot: 100% identical to PIMT_RHIME: Protein-L-isoaspartate O-methyltransferase (pcm) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K00573, protein-L-isoaspartate(D-aspartate) O-methyltransferase [EC: 2.1.1.77] (inferred from 99% identity to smk:Sinme_1336)

Predicted SEED Role

"Protein-L-isoaspartate O-methyltransferase (EC 2.1.1.77)" in subsystem Ton and Tol transport systems (EC 2.1.1.77)

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.77

Use Curated BLAST to search for 2.1.1.77

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See O08249 at UniProt or InterPro

Protein Sequence (204 amino acids)

>SMc02062 protein-L-isoaspartate O-methyltransferase (Sinorhizobium meliloti 1021)
MALRLRSGGIVNHELLKAVEQTPRTLFAPPQYQDEVYSKRLIPLECGSFMEGCDMAVRLL
HCLNLKPGQRILEVGTGSGFTAAVMGRIAERVLTIDRYQTLVASAQKNLEKAGLRNVVVR
QADGSAGVPGEGTFDRILITAAFNSLPRTFSDHLVSGGTLLVPIMMSETHCRIVRVNRTG
SRFDREDLFDAPYLPIVPQVASFL