Protein Info for SMc01866 in Sinorhizobium meliloti 1021

Annotation: UDP-diphospho-muramoylpentapeptide beta-N-acetylglucosaminyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 374 signal peptide" amino acids 1 to 25 (25 residues), see Phobius details TIGR01133: undecaprenyldiphospho-muramoylpentapeptide beta-N-acetylglucosaminyltransferase" amino acids 5 to 354 (350 residues), 276.2 bits, see alignment E=1.9e-86 PF03033: Glyco_transf_28" amino acids 6 to 142 (137 residues), 119.3 bits, see alignment E=2.1e-38 PF13579: Glyco_trans_4_4" amino acids 21 to 137 (117 residues), 33.1 bits, see alignment E=1e-11 PF04101: Glyco_tran_28_C" amino acids 187 to 351 (165 residues), 126.9 bits, see alignment E=1.3e-40

Best Hits

Swiss-Prot: 100% identical to MURG_RHIME: UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase (murG) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K02563, UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase [EC: 2.4.1.227] (inferred from 100% identity to sme:SMc01866)

Predicted SEED Role

"UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase (EC 2.4.1.227)" in subsystem Peptidoglycan Biosynthesis (EC 2.4.1.227)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.4.1.227

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q92NL9 at UniProt or InterPro

Protein Sequence (374 amino acids)

>SMc01866 UDP-diphospho-muramoylpentapeptide beta-N-acetylglucosaminyltransferase (Sinorhizobium meliloti 1021)
MDKGIILLAAGGTGGHLFPAEALAHELKATGYSVHLVTDSRAERFTGKFPADEIHVVPSA
TIGSKNPVKLARSVWKLWTGLRAARRLIARLKPRAVVGFGGYPTVPPLLAATGMGIPSII
HEQNAVMGRANKMLASRVKAVAGGFLPEGTGAFAAKTVATGNPVRPAVLKAAGVPYAPAA
GDAPFHLVVFGGSQGAQFFSKAVPQAVCRLDDALRQRLKVTQQARPEDREGVIAVYEKLG
VPAEVSPFFTDMAGRIASAQLLICRSGASTVSEISVIGRPAILVPYPYALDHDQAANAAA
LAAKGGARVIAQVELSAERLAGILADAMSNPDALAQMAAGARQTGKPDAARLLALLVEAI
ASGSTVAKFKETRS