Protein Info for SMc01864 in Sinorhizobium meliloti 1021
Annotation: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 100% identical to MURD_RHIME: UDP-N-acetylmuramoylalanine--D-glutamate ligase (murD) from Rhizobium meliloti (strain 1021)
KEGG orthology group: K01925, UDP-N-acetylmuramoylalanine--D-glutamate ligase [EC: 6.3.2.9] (inferred from 100% identity to smk:Sinme_2158)Predicted SEED Role
"UDP-N-acetylmuramoylalanine--D-glutamate ligase (EC 6.3.2.9)" in subsystem Peptidoglycan Biosynthesis (EC 6.3.2.9)
MetaCyc Pathways
- peptidoglycan biosynthesis I (meso-diaminopimelate containing) (11/12 steps found)
- UDP-N-acetylmuramoyl-pentapeptide biosynthesis I (meso-diaminopimelate containing) (8/8 steps found)
- UDP-N-acetylmuramoyl-pentapeptide biosynthesis II (lysine-containing) (7/8 steps found)
- peptidoglycan biosynthesis III (mycobacteria) (11/15 steps found)
- peptidoglycan biosynthesis II (staphylococci) (12/17 steps found)
- peptidoglycan biosynthesis IV (Enterococcus faecium) (12/17 steps found)
- peptidoglycan biosynthesis V (β-lactam resistance) (11/17 steps found)
KEGG Metabolic Maps
Isozymes
No predicted isozymesUse Curated BLAST to search for 6.3.2.9
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See Q52953 at UniProt or InterPro
Protein Sequence (463 amino acids)
>SMc01864 UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase (Sinorhizobium meliloti 1021) MIPVTSFKGRKVALFGLGGSGLATAQALVSGGADVVAWDDNPDSVAKAAAAGIATADLRG ADWHAFAAFVLSPGVPLTHPKPHWSVDLAHQAGVEIIGDVELFVRERRKHAPDCPFIAIT GTNGKSTTTALIAHILRTSGRDTQLGGNIGTAVLTLDPPKAGRFYVVECSSYQIDLAPTL DPTAGILLNLTPDHLDRHGTMQHYADIKERLVAGSGTAVVGVDDSLSSLIADRVERAGTK VVRISRRHPLAEGIYAEGSALMRAQDGASSLFTDLAGIQTLRGGHNAQNAAAAIAACLAV GISGKDIVDGLRSFPGLKHRMQPVAKKGEVVFVNDSKATNAEAAAPALSSYDRIYWIAGG LPKEGGITSLAPFFPKIVKAYLIGEAAPSFAATLGEAVPYEISGTLEKAVAHAAADAARD SQGPAAVMLSPACASFDQYKNFEVRGDAFVGHVAALEGVSMLI