Protein Info for SMc01620 in Sinorhizobium meliloti 1021
Annotation: glycerol-3-phosphate dehydrogenase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 67% identical to ERYB_BRUA2: D-erythritol 1-phosphate dehydrogenase (eryB) from Brucella abortus (strain 2308)
KEGG orthology group: K00111, glycerol-3-phosphate dehydrogenase [EC: 1.1.5.3] (inferred from 100% identity to smk:Sinme_2212)MetaCyc: 67% identical to D-erythritol 1-phosphate dehydrogenase (Brucella abortus)
RXN-17768 [EC: 1.1.1.402]
Predicted SEED Role
"Erythritol phosphate dehydrogenase EryB" in subsystem Erythritol utilization
MetaCyc Pathways
- erythritol degradation I (6/6 steps found)
- glycerol-3-phosphate shuttle (2/2 steps found)
- glycerol-3-phosphate to cytochrome bo oxidase electron transfer (2/2 steps found)
- glycerophosphodiester degradation (2/2 steps found)
- glycerol and glycerophosphodiester degradation (3/4 steps found)
- sn-glycerol 3-phosphate anaerobic respiration (2/3 steps found)
- glycerol degradation I (2/3 steps found)
- glycerol 3-phosphate to cytochrome aa3 oxidase electron transfer (1/2 steps found)
- glycerol-3-phosphate to fumarate electron transfer (1/2 steps found)
- glycerol-3-phosphate to hydrogen peroxide electron transport (1/2 steps found)
- nitrate reduction IX (dissimilatory) (1/2 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 1.1.5.3
Use Curated BLAST to search for 1.1.1.402 or 1.1.5.3
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See Q92NH3 at UniProt or InterPro
Protein Sequence (504 amino acids)
>SMc01620 glycerol-3-phosphate dehydrogenase (Sinorhizobium meliloti 1021) MSSETGTYDLFVIGGGINGAGIARDAAGRGLSVLLCEKDDLAQGTSSRSGKLVHGGLRYL EYYEFRLVREALIEREVLLESAPHIIWPMRFVLPHNPADRPAWLVRLGLFLYDHLGGRKR LPGTRVLNLRTAPEGAAIKPAYRKAFEYSDCWVDDARLVVLNALDAKKKGARILTRTACT SIRRRGDLWHVEMTDAETGAKTEAKARCVVNTAGPWVNDVIGRVAGLNSSRSVRLVKGSH IVVPKFWEGRQAYLVQNPDKRVIFINPYQNDLALIGTTDIPYDGRPEDVTADENEVAYLL KSVNRYFKQQLAPGDILHSFSGVRPLYDDNAENPSAVTRDYIFELDAAGKEAPLLSVFGG KITTFRKLSEHALERLRPFFPTMGPAWTARAHLPGGDMADADFDQFLGELRARYRWLPAD LAKHYARLYGTRAHDLIGGATSLDELGTAFTPLFREREARFLIENEWARTAEDLIERRTK HGLHMSEAEKRAFSGWFEGQQAAA