Protein Info for SMc01613 in Sinorhizobium meliloti 1021

Annotation: ribose-5-phosphate isomerase B

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 145 TIGR00689: sugar-phosphate isomerase, RpiB/LacA/LacB family" amino acids 3 to 141 (139 residues), 124 bits, see alignment E=2.2e-40 PF02502: LacAB_rpiB" amino acids 3 to 138 (136 residues), 149.3 bits, see alignment E=3.4e-48

Best Hits

Swiss-Prot: 61% identical to ERYI_BRUA2: D-erythrulose-4-phosphate isomerase (eryI) from Brucella abortus (strain 2308)

KEGG orthology group: K01808, ribose 5-phosphate isomerase B [EC: 5.3.1.6] (inferred from 100% identity to smk:Sinme_2205)

MetaCyc: 61% identical to D-erythrulose-4-phosphate isomerase (Agrobacterium radiobacter K84)
RXN-17762 [EC: 5.3.1.34]

Predicted SEED Role

"Ribose 5-phosphate isomerase B (EC 5.3.1.6)" in subsystem Calvin-Benson cycle or D-ribose utilization or LMPTP YwlE cluster or Pentose phosphate pathway (EC 5.3.1.6)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 5.3.1.6

Use Curated BLAST to search for 5.3.1.34 or 5.3.1.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q92NH9 at UniProt or InterPro

Protein Sequence (145 amino acids)

>SMc01613 ribose-5-phosphate isomerase B (Sinorhizobium meliloti 1021)
MKIAIGADSAGKPLLDVIAAHLAKRSDLEVKDLSQTGYYADLSRDLAQTITAGENERGIL
ICGTGIGVCISANKVPGIRAALTHDTYSAERAAKSNNAQIITMGARVIGPELAKSIVDTW
LASEFDPNGPSAANVEAIDRLDQAK