Protein Info for SMc01532 in Sinorhizobium meliloti 1021

Annotation: alpha-glycosylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 547 PF00128: Alpha-amylase" amino acids 34 to 384 (351 residues), 295.8 bits, see alignment E=5.7e-92 PF16657: Malt_amylase_C" amino acids 473 to 513 (41 residues), 27.6 bits, see alignment 2.5e-10

Best Hits

Swiss-Prot: 65% identical to AGLA_RHIME: Probable alpha-glucosidase (aglA) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K01187, alpha-glucosidase [EC: 3.2.1.20] (inferred from 100% identity to sme:SMc01532)

Predicted SEED Role

"Maltodextrin glucosidase (EC 3.2.1.20)" in subsystem Maltose and Maltodextrin Utilization (EC 3.2.1.20)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.20

Use Curated BLAST to search for 3.2.1.20

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q92N32 at UniProt or InterPro

Protein Sequence (547 amino acids)

>SMc01532 alpha-glycosylase (Sinorhizobium meliloti 1021)
MPAASGSDENWWRGAVIYQVYPRSFQDTDGDGMGDLRGVTRRLPHIASLGVDAIWLSPFF
TSPQADMGYDVSDYCDVDPMFGTLADFDEMLAEAHRLGLKVIIDQVISHTSDRHPWFVES
RSSRTNAKADWYVWADPKPDGTAPNNWLSIFGGPGWEWDGVRRQYYLHNFLSSQPDLNFH
NPEVQEAVLATVRFWLDRGVDGFRLDTANFYFHDRLLRDNPPLVPDPDATSRDAPEVNPY
GMQDHLYDKTQPENLDFLRRFRAVLDEYGGRATVGEVGDGSRSLQTVAAYTSGGDKLHMC
YTFDLLGPEFTARHFRRCVENFQSTVTDGWVCWAFSNHDVMRHVSRFALREADRERVAKL
AISLLASLRGTICLYQGEELGLPEAELAFEELRDPYGIRFWPAFAGRDGCRTPMVWEREL
SNAGFSAGTPWLPVRDGHRMLAVDAQEGVEGAVLEHYRQTLGFRRAQSALVDGDMVFLGT
NQDLLVFTREKDDERLLFVFNLTQEPQTFHLPATLVAEPHPMPGFAPVFADNAIKLAALD
VFCGRLR