Protein Info for SMc01455 in Sinorhizobium meliloti 1021

Annotation: D-lactate dehydrogenase (cytochrome) protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01565: FAD_binding_4
  • amino acids 53 to 189 (137 residues), 129 bits, E=1.1e-41
• PF02913: FAD-oxidase_C
  • amino acids 227 to 466 (240 residues), 221.1 bits, E=2e-69

Best Hits

Swiss-Prot: 48% identical to LDHD_MOUSE: Probable D-lactate dehydrogenase, mitochondrial (Ldhd) from Mus musculus

KEGG orthology group: K00004, (R,R)-butanediol dehydrogenase / diacetyl reductase [EC: 1.1.1.303 1.1.1.4] (inferred from 100% identity to sme:SMc01455)

Predicted SEED Role

"D-Lactate dehydrogenase, cytochrome c-dependent (EC 1.1.2.4)" in subsystem Glycolate, glyoxylate interconversions or Photorespiration (oxidative C2 cycle) (EC 1.1.2.4)

MetaCyc Pathways

• methylglyoxal degradation VIII (3/3 steps found)
• pyruvate fermentation to (R)-acetoin I (3/3 steps found)
• superpathway of (R,R)-butanediol biosynthesis (4/5 steps found)
• (R,R)-butanediol biosynthesis (1/1 steps found)
• (R,R)-butanediol degradation (1/1 steps found)
• methylglyoxal degradation VI (1/4 steps found)

KEGG Metabolic Maps

• Butanoate metabolism
• Pyruvate metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.1.303 or 1.1.1.4 or 1.1.2.4

Sequence Analysis Tools

See Q92NP8 at UniProt or InterPro

Protein Sequence (468 amino acids)

>SMc01455 D-lactate dehydrogenase (cytochrome) protein (Sinorhizobium meliloti 1021)
MALNAIRAGARNENGIAAAKTYLATRFGDRFQTGEAIRAQHANTTTYIPAQLPDAVVFPE
SAAEVREIVEIACEHRVPLIPFGTGSSLEGHVNAPHGGISVDMMRMNRVLAVNAEDLDCT
VEPGVTREELNSYLRDTGLFFPIDPGANASIGGMASTRASGTNAVRYGTMKENVLAVTAV
VAGGREIRTAHRARKSSAGYDLTRLFVGAEGTLGIVTSITLRLQGIPEVISGGVCPFPTI
ADACNAVILTIQSGIPVARIELLDALQMKACNAYSGLTYQETPTLFVEFHGNGESVELQS
RQFAEIASEFGSTGFIWTTNPEERARLWKARHNAYWAQKSLMPGRAILSTDVCVPISRLA
DCVAATHEDIAAHGLVAPIVGHAGDGNFHVGLLFDDKDAADVAQAEAFVERLNARALSMD
GTCTGEHGIGQGKMPFLAAELGDALDLMRQIKRSLDPDNIFNPGKIFA