Protein Info for SMc01441 in Sinorhizobium meliloti 1021

Annotation: membrane bound protease

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 362 transmembrane" amino acids 61 to 84 (24 residues), see Phobius details PF12221: HflK_N" amino acids 17 to 59 (43 residues), 33.1 bits, see alignment 4.4e-12 TIGR01933: HflK protein" amino acids 81 to 350 (270 residues), 285.6 bits, see alignment E=1.8e-89 PF01145: Band_7" amino acids 83 to 262 (180 residues), 113.7 bits, see alignment E=1e-36

Best Hits

KEGG orthology group: K04088, membrane protease subunit HflK [EC: 3.4.-.-] (inferred from 100% identity to smk:Sinme_2103)

Predicted SEED Role

"HflK protein" in subsystem Hfl operon

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.4.-.-

Use Curated BLAST to search for 3.4.-.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q92NQ8 at UniProt or InterPro

Protein Sequence (362 amino acids)

>SMc01441 membrane bound protease (Sinorhizobium meliloti 1021)
MPWSNQNGGGGGPWGGGGGNQGPWGQGPNRPRGGRGGPPDLEEIIRRGQDQLKNVVPGGF
NGGIFVIVGLLILGFVLLNSIYTVQPDERGVEMRFGKPKEEISMPGLHYHFWPLETVEIV
KVTEQQQNIGGRTGQSNAGLMLSGDQNIVNVQFSVLFSVTDPKAYLFNVENPADTLQQVA
ESAMREVVGRRPAQDIFRDNRQAIAADVKNTIQATMDSYGAGISVNTVAIEDAAPPREVA
DAFDEVQRAEQDEDRFVEEANQYANQVLGRARGQGAQIREEAAAYKDRVVKEAQGEAQRF
ISVYDEYSKAPEVTRKRLYIETLQGVLGKSKKVILDEKNGQGVLPYLPLNEIGRPVQSGG
NQ