Protein Info for SMc01364 in Sinorhizobium meliloti 1021

Annotation: DNA topoisomerase I

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 PF01751: Toprim" amino acids 9 to 120 (112 residues), 63.7 bits, see alignment E=3e-21 TIGR01051: DNA topoisomerase I" amino acids 10 to 647 (638 residues), 767.9 bits, see alignment E=6e-235 PF01131: Topoisom_bac" amino acids 136 to 567 (432 residues), 414.7 bits, see alignment E=9.5e-128 PF01396: zf-C4_Topoisom" amino acids 613 to 648 (36 residues), 49.8 bits, see alignment (E = 4.6e-17) PF13368: Toprim_C_rpt" amino acids 669 to 722 (54 residues), 55.1 bits, see alignment 1.3e-18 amino acids 727 to 779 (53 residues), 59 bits, see alignment 7.3e-20 amino acids 796 to 846 (51 residues), 73.8 bits, see alignment 1.7e-24

Best Hits

Swiss-Prot: 49% identical to TOP1_RICFE: DNA topoisomerase 1 (topA) from Rickettsia felis (strain ATCC VR-1525 / URRWXCal2)

KEGG orthology group: K03168, DNA topoisomerase I [EC: 5.99.1.2] (inferred from 100% identity to sme:SMc01364)

Predicted SEED Role

"DNA topoisomerase I (EC 5.99.1.2)" in subsystem Conserved gene cluster associated with Met-tRNA formyltransferase or DNA topoisomerases, Type I, ATP-independent or pVir Plasmid of Campylobacter (EC 5.99.1.2)

Isozymes

Compare fitness of predicted isozymes for: 5.99.1.2

Use Curated BLAST to search for 5.99.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q92QL9 at UniProt or InterPro

Protein Sequence (900 amino acids)

>SMc01364 DNA topoisomerase I (Sinorhizobium meliloti 1021)
MFQRMSMNVVVVESPSKAKTINKYLGPGYKVLASFGHVRDLPAKDGSVRPDEDFEMSWEV
DGASAKRMKDIADAVKSSDGLILATDPDREGEAISWHVLDLLRKKKVIGDKPVKRVVFNA
ITKKAVLDAMAEPRDIDASLVDAYLARRALDYLVGFNLSPVLWRKLPGARSAGRVQSVAL
RLVCDREAEIERFVTEEYWNISALLKTPRGDEFEARLVSADGKRLPPKAIGNGEEANRLK
SLLDGASYVVESVEAKPVKRNPSPPFTTSTLQQAASSKLGFSASRTMQVAQKLYEGVDIG
GETVGLITYMRTDGVQMAPEAIEAARQAIGSQFGERYLPEKPRFYSTKAKNAQEAHEAIR
PTDFDRTPDQVRRFLDGDMLRLYDLVWKRGIASQMASAEIERTTAEIVADNAGKKAGLRA
TGSVIRFDGFIAAYTDMKEDGEQADDGDEDGRLPEINARENLAKQKINASQHFTEPPPRY
SEATLIKKMEELGIGRPSTYAATVTTLIDRDYVEIDKRKLVPQAKGRLVTAFLESFFTRY
VEYDFTASLEEKLDQISAGELNWKDVLRDFWKDFFSQIEDTKELRVTNVLDALNEELAPL
VFPKREDGGDPRICQVCGTGKLSLKLGKYGAFVGCSNYPECNYTRQLSSDSNGDAEAAAA
NEPQSLGKDPHTGEEITLRNGRFGPYVQRGDGKEAKRASLPKGWTPAAIDHEKALALLSL
PRDIGPHPESGKMISTGIGRYGPFVLHNGTYANLESVEDVFSIGLNRAISVLADKQSKGA
GGGRASAAALKELGEHPDGGAITVRDGRFGPYVNWGKVNATLPRGKDPQSVTVEEALALI
AEREAKGGVTKGKAAKGKPTGGKSAGTKSAKAASAGTAKAEKPKSAAKTKTKAAAKAKKD