Protein Info for SMc01361 in Sinorhizobium meliloti 1021

Annotation: dihydroorotase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 430 TIGR00857: dihydroorotase, multifunctional complex type" amino acids 24 to 428 (405 residues), 327.7 bits, see alignment E=5.5e-102 PF01979: Amidohydro_1" amino acids 244 to 427 (184 residues), 42.1 bits, see alignment E=6.7e-15 PF07969: Amidohydro_3" amino acids 348 to 428 (81 residues), 32.4 bits, see alignment E=7.7e-12

Best Hits

Swiss-Prot: 39% identical to PYRC_EHRCJ: Dihydroorotase (pyrC) from Ehrlichia canis (strain Jake)

KEGG orthology group: K01465, dihydroorotase [EC: 3.5.2.3] (inferred from 100% identity to smk:Sinme_1099)

Predicted SEED Role

"Dihydroorotase (EC 3.5.2.3)" in subsystem De Novo Pyrimidine Synthesis (EC 3.5.2.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.5.2.3

Use Curated BLAST to search for 3.5.2.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q92QL6 at UniProt or InterPro

Protein Sequence (430 amino acids)

>SMc01361 dihydroorotase (Sinorhizobium meliloti 1021)
MTRPLVLQNLRIVDPSRKLDEIGTIIVGDDGRILAAGKDAQNQGVPADAFVKDCAGLTAV
PGLVDARVFVGEPGSEHRETIESASRAAATGGVTSFIAMPDTDPVIDDIALVEFVQKTAR
DKALVNVHPAAALTKGLLGQEMTEIGLLRDAGAVCFTNGRQPVHDTLVLRRAMTYAREFG
AVVALGTRDKYLGANGVMNEGLLASWLGLPGVPREAEIIPLERDLRIAGLTRAAYHAAEI
SVPESAETIRIARERGTNVTCGISINHLTLNENDIGEYRTFFKLSPPLRTEDDRVAMVEA
LADGTIDIIVSSHDPQDVDTKRLPFADAADGAIGLETLAAAALRLYHSGEVPLMRLIDAL
STRPSAIFGLDAGTLKPGARADIAVIDLDEPWVLHKETLVSRSKNTPFENARFTGRVVQT
YVAGELVHSA