Protein Info for SMc01327 in Sinorhizobium meliloti 1021

Annotation: tRNA/rRNA methyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 291 PF08032: SpoU_sub_bind" amino acids 59 to 131 (73 residues), 44.4 bits, see alignment E=1.7e-15 PF00588: SpoU_methylase" amino acids 143 to 283 (141 residues), 119.9 bits, see alignment E=9.4e-39

Best Hits

KEGG orthology group: K03218, RNA methyltransferase, TrmH family [EC: 2.1.1.-] (inferred from 100% identity to sme:SMc01327)

Predicted SEED Role

"23S rRNA (guanosine-2'-O-) -methyltransferase rlmB (EC 2.1.1.-)" (EC 2.1.1.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.-

Use Curated BLAST to search for 2.1.1.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q92QI6 at UniProt or InterPro

Protein Sequence (291 amino acids)

>SMc01327 tRNA/rRNA methyltransferase (Sinorhizobium meliloti 1021)
MSKDKTGDKSARDTHYANLRRAHRDARRERGEIPTPRQDKRAKLSADWKPPALAPDQVLL
YGLHTVRAALDNPERRIIRLSVTQNAAARLDLGDLTALPFPTETVMPQELDRILGPDAIH
QGVMLETQPLPHRKLEALRDCPLILVLDQVTDPHNVGAIMRSAVAFDAGALITTMRHSPT
ESGVLAKSASGALELIPYIQITNLADTLEELHELGFMTIGLDSEGPQPLEGTFAGDKIAL
VLGSEGKGLRQKTRQTVKALARLDMPGAIKSLNVSNAAAIAMYAARHHLKG