Protein Info for SMc01275 in Sinorhizobium meliloti 1021

Annotation: ribosomal large subunit pseudouridine synthase C protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00005: pseudouridine synthase, RluA family
  • amino acids 12 to 315 (304 residues), 211.2 bits, E=1.1e-66
• PF00849: PseudoU_synth_2
  • amino acids 104 to 257 (154 residues), 127.3 bits, E=3.1e-41

Best Hits

KEGG orthology group: K06179, ribosomal large subunit pseudouridine synthase C [EC: 5.4.99.12] (inferred from 100% identity to smk:Sinme_1183)

Predicted SEED Role

"Ribosomal large subunit pseudouridine synthase C (EC 4.2.1.70)" in subsystem Ribosome biogenesis bacterial (EC 4.2.1.70)

MetaCyc Pathways

• pseudouridine degradation (1/2 steps found)

KEGG Metabolic Maps

• Pyrimidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.70, 5.4.99.12

Use Curated BLAST to search for 4.2.1.70 or 5.4.99.12

Sequence Analysis Tools

See Q92QE2 at UniProt or InterPro

Protein Sequence (330 amino acids)

>SMc01275 ribosomal large subunit pseudouridine synthase C protein (Sinorhizobium meliloti 1021)
MAGIEHRQVDSDEAGMRLDRWFKVHFPGLGFGPLQKLLRSGQIRVDGARAKSDTRIQPGQ
TIRIPPLGVDPKEAKSGPIGGRDLRQSPDGELLSRMVLHEDAKVIVLNKPAGLAVQGGSG
VNRHIDKMLEAWTSQKGEKPRLVHRLDRDTSGVLVVARTRGAAQQLTAAFRERDTKKTYW
ALVRGVPRKREDKISTWLVKEPTPDGDRVRIAKHGEDGADHAISYYRIVEQAGQNLAWLE
MEPYTGRTHQLRVHAAHIGHPIIGDPKYFEADVNWTFPGGMQNRLHLHARHIDIPHPNGG
RLKITAPLPQHMVQSWNLLGFDENAAGEEE