Protein Info for SMc01192 in Sinorhizobium meliloti 1021

Annotation: methionyl-tRNA synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 516 PF00133: tRNA-synt_1" amino acids 5 to 221 (217 residues), 41.7 bits, see alignment E=1.4e-14 amino acids 227 to 338 (112 residues), 42.4 bits, see alignment E=8.3e-15 TIGR00398: methionine--tRNA ligase" amino acids 7 to 493 (487 residues), 514.5 bits, see alignment E=1.7e-158 PF09334: tRNA-synt_1g" amino acids 8 to 365 (358 residues), 373.4 bits, see alignment E=2.4e-115 PF19303: Anticodon_3" amino acids 377 to 508 (132 residues), 42.9 bits, see alignment E=9.4e-15

Best Hits

Swiss-Prot: 100% identical to SYM_RHIME: Methionine--tRNA ligase (metG) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K01874, methionyl-tRNA synthetase [EC: 6.1.1.10] (inferred from 100% identity to sme:SMc01192)

Predicted SEED Role

"Methionyl-tRNA synthetase (EC 6.1.1.10)" (EC 6.1.1.10)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.10

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q92PX0 at UniProt or InterPro

Protein Sequence (516 amino acids)

>SMc01192 methionyl-tRNA synthetase (Sinorhizobium meliloti 1021)
MRDTSPFYITTAISYPNGKPHIGHAYELIATDAMARFQRLDGREVFFLTGTDEHGQKMQQ
TAKKEGISPQELAARNSAEFQNMARLLNASNDDFIRTTEQRHHEASQAIWMRMGEAGDLY
KDSYAGWYSVRDEAYYQENETELRGDGVRYGPQGTPVEWVEEESYFFRLSAYQDKLLKHY
EENPDFIGPAERRNEVISFVKSGLKDLSVSRTTFDWGIKVPNDPSHVMYVWVDALTNYVT
ATGCLTDPTGPRAKFWPANIHVIGKDIIRFHAVYWPAFLMSAGLPLPKRVFAHGFLLNKG
EKMSKSLGNVVDPFNLVEHFGLDQIRYFFLREVSFGQDGSYSEEGIATRINSDLANGIGN
LASRSLSMIVKNCDGQVPLCGPLTDEDKAMLAAADSLIGTAREEMGKQLIHRALAAIIAV
VSETDRYFAGQEPWALKKTDPSRMATVLYVTAEVVRQVAILLQPFMPESAGKLLDLVAVP
TDRRDFAHLGEAGRLVSGTPLEAPKPVFPRYVAPEA