Protein Info for SMc01172 in Sinorhizobium meliloti 1021

Annotation: thiosulfate sulfurtransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 40% identical to THTM_HUMAN: 3-mercaptopyruvate sulfurtransferase (MPST) from Homo sapiens

KEGG orthology group: K01011, thiosulfate/3-mercaptopyruvate sulfurtransferase [EC: 2.8.1.1 2.8.1.2] (inferred from 100% identity to sme:SMc01172)

MetaCyc: 40% identical to 3-mercaptopyruvate sulfurtransferase (Homo sapiens)
3-mercaptopyruvate sulfurtransferase. [EC: 2.8.1.2]; 2.8.1.- [EC: 2.8.1.2]

Predicted SEED Role

"3-mercaptopyruvate sulfurtransferase (EC 2.8.1.2)" (EC 2.8.1.2)

MetaCyc Pathways

thiosulfate disproportionation IV (rhodanese) (1/1 steps found)
L-cysteine degradation III (1/2 steps found)
tRNA-uridine 2-thiolation (mammalian mitochondria) (2/4 steps found)
tRNA-uridine 2-thiolation (yeast mitochondria) (2/4 steps found)
sulfide oxidation IV (mitochondria) (2/5 steps found)
tRNA-uridine 2-thiolation (cytoplasmic) (2/8 steps found)

KEGG Metabolic Maps

Cysteine metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.8.1.1, 2.8.1.2

Use Curated BLAST to search for 2.8.1.1 or 2.8.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q92PV4 at UniProt or InterPro

Protein Sequence (287 amino acids)

>SMc01172 thiosulfate sulfurtransferase (Sinorhizobium meliloti 1021)
MNGENENKSRFVVSADWLEQRLDDPSVKIIDAAWYLPAQNRDAKAEYAAAHIPGAVFFDQ
DAIANRTSALPHTLPSPEAFSEAVGAMGISENDTIVVYDGPGIFTAPRVWWMFRIMGAEN
VFVLDGGMDGWKADGRPTTTEVAKPSPQAFNAVFNPNAVTSFERMRDVVEHRLSQIADAR
SAGRFAGEEPEPRAGMRSGHMPGARSLPSGVFSDKGRFKDLDALRRTFADAGIDLTKPVV
TSCGSGITAAIITLALQSLGHKDNTLYDGSWSEWGSRPDTPVAIGKE