Protein Info for SMc01166 in Sinorhizobium meliloti 1021

Updated annotation (from data): 3D-(3,5/4)-trihydroxycyclohexane-1,2-dione hydrolase IolD (EC 3.7.1.22)
Rationale: Specifically important for utilizing m-Inositol.
Original annotation: malonic semialdehyde oxidative decarboxylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 617 TIGR04377: 3,5/4-trihydroxycyclohexa-1,2-dione hydrolase" amino acids 7 to 615 (609 residues), 952.4 bits, see alignment E=5.9e-291 PF02776: TPP_enzyme_N" amino acids 49 to 132 (84 residues), 40.8 bits, see alignment E=2.6e-14 PF00205: TPP_enzyme_M" amino acids 222 to 354 (133 residues), 137.5 bits, see alignment E=3.9e-44 PF02775: TPP_enzyme_C" amino acids 420 to 571 (152 residues), 108.2 bits, see alignment E=5.3e-35

Best Hits

KEGG orthology group: K03336, 3D-(3,5/4)-trihydroxycyclohexane-1,2-dione hydrolase [EC: 3.7.1.-] (inferred from 100% identity to sme:SMc01166)

Predicted SEED Role

"Epi-inositol hydrolase (EC 3.7.1.-)" in subsystem Inositol catabolism (EC 3.7.1.-)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.7.1.- or 3.7.1.22

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q92SL8 at UniProt or InterPro

Protein Sequence (617 amino acids)

>SMc01166 3D-(3,5/4)-trihydroxycyclohexane-1,2-dione hydrolase IolD (EC 3.7.1.22) (Sinorhizobium meliloti 1021)
MSQKTVRLTMAQAVARFLTRQMTIIDGERVPIFGGVFAIFGHGNVAGVGEALYGVRETLP
TYRAQNEQGMANAAIAFAKASFRRRFMACTTSIGPGALNMVTSAALAHVNRLPVLLLPGD
VFANRRPDPVLQQVESFGDGTISANDCFKPVSRYFDRITRPEQIIPALRRAMQVLTDPAD
CGPVTLSLCQDVQAEAYDYPESFFDEKVWVPRRIEPDLDELASAIEALRSAKKPIIIAGG
GVLYSEASAELAAFAEKHGIPVVETQAGKSALPHSHPLNMGSVGVTGTSASNALAEEADV
VLAVGSRLQDFTTGSWALFKNEALKIIGLNVQAFDAGKHEGQPLISDARAGLNRISGGLG
SYKADSAWTERAKAGKAEWLAAAERATATTNAALPSDAQVIGAVQRARGGRKTTLVCAAG
GLPGELHKLWQAEEPGGYHMEYGFSTMGYEVAGGLGVKLAKPDSDVIVMVGDGSYMMLNS
EIASSIMLGAKFTVVLLDNAGYGCINRLQMGTGGANFNNLLKDTHHVELPQIDFAAHAAA
MGAVTRKVGSIPELEAALAETADEVRTTVIVIDTDPLITTEAGGHWWDVAVPEVSDRDQV
KAAREGYEKALQAQRFG