Protein Info for SMc01161 in Sinorhizobium meliloti 1021

Annotation: bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 401 signal peptide" amino acids 6 to 18 (13 residues), see Phobius details TIGR00521: phosphopantothenoylcysteine decarboxylase / phosphopantothenate--cysteine ligase" amino acids 4 to 393 (390 residues), 410 bits, see alignment E=5.3e-127 PF02441: Flavoprotein" amino acids 6 to 179 (174 residues), 120.8 bits, see alignment E=4.3e-39 PF04127: DFP" amino acids 187 to 371 (185 residues), 228.8 bits, see alignment E=4.8e-72

Best Hits

Swiss-Prot: 50% identical to COABC_VIBVU: Coenzyme A biosynthesis bifunctional protein CoaBC (coaBC) from Vibrio vulnificus (strain CMCP6)

KEGG orthology group: K13038, phosphopantothenoylcysteine decarboxylase / phosphopantothenate--cysteine ligase [EC: 4.1.1.36 6.3.2.5] (inferred from 100% identity to sme:SMc01161)

MetaCyc: 47% identical to fused 4'-phosphopantothenoylcysteine decarboxylase and phosphopantothenoylcysteine synthetase (Escherichia coli K-12 substr. MG1655)
Phosphopantothenate--cysteine ligase. [EC: 6.3.2.5]; Phosphopantothenoylcysteine decarboxylase. [EC: 6.3.2.5, 4.1.1.36]

Predicted SEED Role

"Phosphopantothenoylcysteine decarboxylase (EC 4.1.1.36) / Phosphopantothenoylcysteine synthetase (EC 6.3.2.5)" in subsystem Coenzyme A Biosynthesis (EC 4.1.1.36, EC 6.3.2.5)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.1.1.36 or 6.3.2.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q92SL3 at UniProt or InterPro

Protein Sequence (401 amino acids)

>SMc01161 bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate synthase (Sinorhizobium meliloti 1021)
MTLSGKRILLIISGGIAAYKSLDLIRRLRERGADVRPVMTAAAQRFVTPLTVGAISASHV
FTELFAREDEQDVGHIRLARHCDLVVVAPATADLMAKMAHGHADDLASTVLLATDRPVLL
APAMNPRMWSHPATLRNRATLAGDGVRFVGPATGEMAESGEAGEGRMAEPLEIVAAVEEL
FSEARPLAGRKAIVTSGPTHEPIDPVRYIANRSSGKQGHAIAAALARLGAEVTLVSGPVT
IADPAGVRVVRVERAEEMRDAVLAALPADVAVMVAAVADWRVASTAGNKIKKRPGEAPPP
LQLAENPDILKTVGHYASRPRLVIGFAAETENVAENGRAKLQKKGADYILANDVSPATGI
MGGDRNRVKLIGRDGTEEWPEMGKDDVAAKLAALIVAKLSS