Protein Info for SMc01153 in Sinorhizobium meliloti 1021

Annotation: enoyl-CoA hydratase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 100% identical to ECHH_RHIME: Probable enoyl-CoA hydratase (fadB1) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K01692, enoyl-CoA hydratase [EC: 4.2.1.17] (inferred from 100% identity to smk:Sinme_3697)

MetaCyc: 65% identical to acryloyl-CoA hydratase monomer (Roseovarius nubinhibens ISM)
RXN-12573 [EC: 4.2.1.155]; RXN-6383 [EC: 4.2.1.155, 4.2.1.116]

Predicted SEED Role

"Enoyl-CoA hydratase [branched-chain amino acid degradation] (EC 4.2.1.17)" (EC 4.2.1.17)

MetaCyc Pathways

oleate β-oxidation (29/35 steps found)
superpathway of glyoxylate cycle and fatty acid degradation (12/14 steps found)
superpathway of coenzyme A biosynthesis II (plants) (9/10 steps found)
adipate degradation (5/5 steps found)
benzoyl-CoA biosynthesis (3/3 steps found)
β-alanine biosynthesis II (5/6 steps found)
acrylate degradation I (4/5 steps found)
adipate biosynthesis (4/5 steps found)
propanoyl-CoA degradation II (4/5 steps found)
fatty acid β-oxidation I (generic) (5/7 steps found)
acrylate degradation II (2/3 steps found)
fatty acid β-oxidation II (plant peroxisome) (3/5 steps found)
fatty acid β-oxidation IV (unsaturated, even number) (3/5 steps found)
2-methyl-branched fatty acid β-oxidation (9/14 steps found)
methyl ketone biosynthesis (engineered) (3/6 steps found)
phenylacetate degradation I (aerobic) (5/9 steps found)
valproate β-oxidation (5/9 steps found)
(8E,10E)-dodeca-8,10-dienol biosynthesis (6/11 steps found)
superpathway of phenylethylamine degradation (6/11 steps found)
benzoyl-CoA degradation I (aerobic) (3/7 steps found)
fatty acid β-oxidation VI (mammalian peroxisome) (3/7 steps found)
3-hydroxypropanoate cycle (7/13 steps found)
benzoate biosynthesis III (CoA-dependent, non-β-oxidative) (1/5 steps found)
3-hydroxypropanoate/4-hydroxybutanate cycle (10/18 steps found)
3-phenylpropanoate degradation (4/10 steps found)
benzoate biosynthesis I (CoA-dependent, β-oxidative) (3/9 steps found)
glyoxylate assimilation (4/13 steps found)
superpathway of the 3-hydroxypropanoate cycle (7/18 steps found)
(4Z,7Z,10Z,13Z,16Z)-docosapentaenoate biosynthesis (6-desaturase) (2/13 steps found)
docosahexaenoate biosynthesis III (6-desaturase, mammals) (2/14 steps found)
Spodoptera littoralis pheromone biosynthesis (4/22 steps found)
platensimycin biosynthesis (6/26 steps found)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.17

Use Curated BLAST to search for 4.2.1.116 or 4.2.1.155 or 4.2.1.17

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q52995 at UniProt or InterPro

Protein Sequence (257 amino acids)

>SMc01153 enoyl-CoA hydratase (Sinorhizobium meliloti 1021)
MSYETLLVETQGRVGLITLNRPQALNALNAVLMRELDAALKAFDADRAVGAIVLAGSEKA
FAAGADIKEMQGLDFVDGYLADFLGGWEHVANARKPMIAAVSGFALGGGCELAMMCDFII
ASETAKFGQPEITLGVIPGMGGSQRLTRAVGKAKAMDLILTGRMMDAAEAERSGLVSRVV
APDRLLEEALGAAEKIASFSLPAAMMAKEAVNRSLELTLAEGLRFERRLFQSLFATEDQK
EGMAAFVAKRKAEFKHR