Protein Info for SMc01124 in Sinorhizobium meliloti 1021

Annotation: PII uridylyl-transferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 949 TIGR01693: protein-P-II uridylyltransferase" amino acids 51 to 915 (865 residues), 1172.1 bits, see alignment E=0 PF01909: NTP_transf_2" amino acids 98 to 176 (79 residues), 28.4 bits, see alignment E=4.4e-10 PF08335: GlnD_UR_UTase" amino acids 209 to 347 (139 residues), 158.7 bits, see alignment E=2.3e-50 PF01966: HD" amino acids 494 to 608 (115 residues), 52.8 bits, see alignment E=1.2e-17 PF01842: ACT" amino acids 844 to 891 (48 residues), 30 bits, see alignment 9.1e-11

Best Hits

Swiss-Prot: 100% identical to GLND_RHIME: Bifunctional uridylyltransferase/uridylyl-removing enzyme (glnD) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K00990, [protein-PII] uridylyltransferase [EC: 2.7.7.59] (inferred from 100% identity to smk:Sinme_0029)

Predicted SEED Role

"[Protein-PII] uridylyltransferase (EC 2.7.7.59)" in subsystem Ammonia assimilation (EC 2.7.7.59)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.7.59

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P56884 at UniProt or InterPro

Protein Sequence (949 amino acids)

>SMc01124 PII uridylyl-transferase (Sinorhizobium meliloti 1021)
MARHETSFPEILDVAALRARCDFIASAHAEQREPMRRALLAAFKEANVAGRAKARELLAA
DGAGIKCAERISWLQDQLITLLHDFVLNQVFDAAKAPEASRIAVTAVGGYGRGTLAPGSD
IDLLFLLPAKKAVWAEPAIEFMLYILWDLGFKVGHATRTIEECIRLSRADMTIRTAILEC
RYVCGSAALANELETRFDHEIVRNTGPEFIAAKLAERDERHRKAGDTRYLVEPNVKEGKG
GLRDLHTLFWISKYFYRVKDSADLVKLGVLSKQEYKLFQKAEDFLWAVRCHMHFLTGKAE
ERLSFDIQREIAEALGYHDHPGLSAVERFMKHYFLVAKDVGDLTRIFCSALEDQQAKDTP
GITGVISRFRNRVRKIAGTLDFVDDGGRIALAGTDVFKRDPVNLMRLFHIADINGLEFHP
AALKQVTRSLGLITPHLRENEEANRLFLSILTSRRNPELILRRMNEAGVLGRFIPEFGKI
VSMMQFNMYHHYTVDEHLLRSVDVLSRIERGLEEEAHPLTAMLMPGIEDREALYVAVLLH
DIAKGRPEDHSVAGAKVARKLCPRFRLTPKQTEMVVWLVEEHLTMSMVAQTRDLNDRKTI
VDFAERVQSLERLKMLLILTVCDIRAVGPGVWNGWKGQLLRTLYYETELLLSGGFSELSR
KERAKHAAHMLEEALADWPKKERQAYVRLHYQPYLLTVALEEQVRHAGFIREADRAGRTL
ATMVRTHDFHAITEITVLSPDHPRLLTVIAGACAAAGANIVGAQIHTTSDGRALDTILVN
REFSVAEDETRRAASIGKLIEDVLSGRKRLPEVIASRTRVKKRSRAFTVTPEVTISNTLS
NKFTVIEVEGLDRTGLLSEVTAVLSDLSLDIASAHITTFGEKVIDTFYVTDLVGSKITSE
NRQMNIAARLKAVLAGEVDEARERMPSGIIAPTPVSRVPHGSKTTKAET